>c-MET / HGFR (aa 956-1390) Protein (GST Tag)
|Catalog||Size (Price)||Quantity||In Stock||Operation||Other Information|
Met proto-oncogene (hepatocyte growth factor receptor) Protein Datasheet
c-MET / HGFR Protein Price Inquiry ( Available Sizes )
c-MET / HGFR Protein Product Information
|Protein Construction:||A DNA sequence encoding the human MET (P08581-1)(Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.|
|Expression Host:||Baculovirus-Insect cells|
c-MET / HGFR Protein QC Testing
|Purity:||> 97 % as determined by SDS-PAGE||SDS-PAGE:
c-MET / HGFR protein
|Measured by its binding ability in a functional ELISA.
Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Met|
The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10%glycerol, 3mM DTT.
c-MET / HGFR Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
c-MET / HGFR Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|HGFR/c-MET||Human||HGFR/c-MET/Fc Protein, Recombinant||10692-H03H|
|HGFR/c-MET||Human||HGFR/c-MET Protein, Recombinant||10692-H08H|
|HGFR/c-MET||Human||c-MET / HGFR (aa 956-1390) Protein, Recombinant, with GST Tag||10692-H20B1|
|HGFR/c-MET||Mouse||HGFR/c-MET/Fc Protein, Recombinant||50622-M02H|
|HGFR/c-MET||Mouse||HGFR/c-MET Protein, Recombinant||50622-M08H|
|HGFR/c-MET||Canine||HGFR/c-MET Protein, Recombinant||70008-D08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||HGFR/c-MET Antibody, Mouse MAb||10692-MM02|
|ELISA, IHC-P||HGFR / c-MET Antibody (Antigen Affinity Purified)||10692-RP02|
|WB, ELISA||HGFR/c-MET Antibody, Rabbit PAb||70008-RP02|
|WB, ELISA||HGFR / c-MET Antibody||70008-MM05|
c-MET / HGFR Protein Description
The proto-oncogene MET encoded product MET (mesenchymal-epithelial transition factor), also known as c-Met or hepatocyte growth factor receptor (HGFR), is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. As the prototypic member of a small subfamily of growth factor receptors, c-Met/HGFR is synthesized as a single chain precursor, and is processed into a mature disulfide-linked heterodimer composed of a extracellular α subunit and a transmembrane β subunit via post-translational cleavage. c-Met/HGFR is identified as a glycosylated receptor tyrosine kinase (RTK), and HGF is the only known ligand. Following ligand binding and autophosphorylation, c-Met/HGFR transmits intercellular signals using a unique multisubstrate docking site which mediates the binding of multiple SH2-containing adapter proteins such as Grb2, SHC, Crk/CRKL, as well as Gab1. Normal c-Met/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active c-Met/HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. Two transcript variants encoding different isoforms have been identified.
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