c-MET / HGFR (aa 956-1390) Protein (GST Tag)

Met proto-oncogene (hepatocyte growth factor receptor) Protein Datasheet

c-MET / HGFR Protein Product Information

• Synonym: MET
• Protein Construction: A DNA sequence encoding the human MET (P08581-1)(Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

c-MET / HGFR Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10%glycerol, 3mM DTT.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: c-MET / HGFR protein

c-MET / HGFR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

c-MET / HGFR Protein Description

The proto-oncogene MET encoded product MET (mesenchymal-epithelial transition factor), also known as c-Met or hepatocyte growth factor receptor (HGFR), is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. As the prototypic member of a small subfamily of growth factor receptors, c-Met/HGFR is synthesized as a single chain precursor, and is processed into a mature disulfide-linked heterodimer composed of a extracellular α subunit and a transmembrane β subunit via post-translational cleavage. c-Met/HGFR is identified as a glycosylated receptor tyrosine kinase (RTK), and HGF is the only known ligand. Following ligand binding and autophosphorylation, c-Met/HGFR transmits intercellular signals using a unique multisubstrate docking site which mediates the binding of multiple SH2-containing adapter proteins such as Grb2, SHC, Crk/CRKL, as well as Gab1. Normal c-Met/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active c-Met/HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. Two transcript variants encoding different isoforms have been identified.

References

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