All c-Kit reagents are produced in house and quality controlled, including 14 c-Kit Antibody, 2 c-Kit ELISA, 27 c-Kit Gene, 1 c-Kit IPKit, 7 c-Kit Lysate, 8 c-Kit Protein, 3 c-Kit qPCR. All c-Kit reagents are ready to use.
Recombinant c-Kit proteins are expressed by Baculovirus-Insect Cells, HEK293 Cells, E. coli with fusion tags as N-GST & His, C-human IgG1-Fc, C-His, N-His.
c-Kitantibodies are validated with different applications, which are FCM, ELISA, ELISA(Cap), ELISA(Det), WB, IP, ICC/IF, IF, IHC-P.
c-KitcDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each c-Kit of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
c-KitELISA Kit are quality controlled by 8 internation QC standard which guarantee every ELISA Kit with high quality.
Expression host: Baculovirus-Insect Cells
C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.