bFGF / FGF2 Antibody (Rabbit Monoclonal Antibody)

Basic Fibroblast Growth Factor ( bFGF / FGF2 ) Antibody

bFGF / FGF2 Antibody Product Information

• Immunogen: Recombinant human bFGF protein ( Catalog #10014-HNAE )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 101
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, E.coli-derived, recombinant human bFGF / FGF2 ( rh FGF2; Catalog#10014-HNAE; NP_001997.5; Pro 143 - Ser 288 ).

bFGF / FGF2 Antibody Usage Guide

• Specificity: Human bFGF / FGF2
• No cross-reactivity in ELISA with
  

  Human aFGF / FGF1

  Human FGF9 / GAF

  Human FGF10 / FGF-10

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human bFGF in WB. Using a DAB detection system, the detection limit for human bFGF is approximately 0.125 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1- 0.2 μg/mL with the appropriate secondary reagents to detect human bFGF. The detection limit for bFGF is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

bFGF / FGF2 Antibody Background

bFGF (FGF basic) is a member of the FGF family consisting of at least 23 related mitogenic proteins and is secreted by mechanisms other than the classical protein secretion pathway due to the lack of signal peptide. bFGF is expressed extensively, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. Members of the fibroblast growth factor (FGF) family of proteins stimulate the proliferation and differentiation of target cells through receptor-mediated pathways, and it appears that FGF acidic and basic bind to the same high affinity receptors. Binding of bFGF to heparin or cell surface heparan sulfate proteoglycans is prerequisite for ligation of bFGF and the high affinity receptors. FGF basic and acidic have a similar range of biological activities which are implicated in several important physiological and pathological processes, such as embryonic development and differentiation, morphogenesis, angiogenesis, and wound healing. Five different isoforms with distinct properties are resulted from alternative translation from non-AUG (CUG) and AUG initiation codons. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

References

1. Wiedlocha, A. et al., 1994, Cell. 76: 1039-51.
2. Orpana, A. et al., 2002, Leuk. Lymphoma. 43: 219-24.
3. Dowd, CJ. et al., 1999, J. Biol. Chem. 274: 5236-44.
4. Lee, M. et al., 2008, Tissue. Eng. Part A. 14:1395-402.