Quantification

1. How do you determine the quantity of your proteins and antibodies?

Normally we determine the concentration of proteins and antibodies by measuring their UV absorbance. The concentration of proteins and antibodies is calculated by their extinction coefficients. We also use BCA, SDS-PAGE, HPLC and other methods to quantify the proteins and antibodies.

2. Why is the quantification of the protein generated by my assay different from the one on the label?

Different assays generate different quantification result. Sometimes the discrepancy can be significant if you conduct a different assay. It is also possible that the protein forms aggregations during storage which causes loss after reconstitution and centrifugation. We run quality control tests for each batch of proteins and antibodies. However, it is still inevitable that a few vials are not the same with the rest of the same batch. This happens very rare and can be minimized by our effort on Quality Control.

3. Who should I report to if I find the product quantity is incorrect? How will you deal with this kind of complaints?

You can call us at +86-400-890-9989 or send us an email to order@sinobiological.com. We will repeat your quantification assay on the same batch of product that was sent to you. If there is a discrepancy between the two assays, we will perform more types of assays to determine the quantity. If the quantity is actually incorrect, we will make up the shortage for free and give you credits for your future orders.