Hybridoma Cell Culture Services

Hybridoma Cell Culture Services

Monoclonal antibody production using hybridoma ascites in rodent such as mice is an old technology and is being widely used, but because of the usage of animal, it is not preferred and has even been banned in certain countries. The preferred method for monoclonal antibody production is hybridoma cell culture or recombinant antibody production in mammalian cells.

Sino Biological has an industry leading technology platform for large scale hybridoma cell culture in stirred tanks. It does not require the use of animals. Moreover, the monoclonal antibodies produced are generally of higher quality, free of contamination of non-specific binding antibodies. Growing various types of hybridoma cell lines is not a simple task. Sino Biological’s serum-free medium formulation and cell culture process scale-up expertise allow us to provide cost-effective services for hybridoma cell culture at various production scale ranging from mg to tens of grams.

To date, Sino Biological has successfully produced many different types of hybridoma cell culture for clients at gram quantity scale. Our success rate is 100% for stable cell lines. We also have technology in place to handle unstable hybridoma cell lines where we could clone the antibody gene out and use our recombinant antibody production technology for transient production in HEK293 or CHO cells. We also have experience in purifying all types of monoclonal antibodies from various species such as mouse, rat, rabbit, and canine.

Hybridoma Cell Culture Related Services and Products:

• Rabbit Monoclonal Antibody Development
• Mouse Monoclonal Antibodies for Influenza Research
• Monoclonal Antibody Purification Platform for Bulk Production
• Monoclonal antibody VL and VH gene cloning