Mab Gene Cloning from Hybridoma

Monoclonal Antibody Gene Cloning Services

Cloning the correct antibody genes from hybridoma cells allows one to produce the antibodies by recombinant technology and switch the antibody constant genes to a different species or different sub-types. It is also essential to produce large quantity of an important antibody from a difficult-to-grow or instable hybridoma cell line.

Cloning the correct mouse or rat antibody genes from a hybridoma cell line is not an easy task to anybody. Extensive experience and expertise in designing primers and analyzing antibody genes is essential to clone the correct genes and sort out pseudo genes.

Sino Biological offers full services from hybridoma development, gene cloning, recombinant antibody production by our state-of-the-art transient transfection technology, to ELISA assay development. We have successfully cloned hundreds of antibodies from a variety of cell lines: mouse hybridoma, rat hybridoma, and rabbit hybridoma cell lines.

We offer chimeric antibody engineering and class switch services free of charge to our customers. Clients can select a specific type of species and sub-class of antibody constant regions for their antibodies to fit a specific purpose. However, it should be note that switching the antibody constant regions could potentially reduce the antibody binding affinity for some antibodies. So it is recommended to match the original antibody’s constant regions for most applications.

Monoclonal Antibody Related Services and Products

• Antibody Development, Production, and Catalog Products.
• Monoclonal Antibody Services.
• Protein Antigen Catalog Products & Production Services.
• Purification Platform.
• Rabbit Monoclonal Antibody Development.
• Polyclonal Antibody Development Services.
• Rabbit Monoclonal Antibody Services.
• Rabbit Monoclonal Antibodies for Influenza Research .
• Monoclonal Antibody Purification Platform for Bulk Production.
• Highest Mouse Monoclonal Antibody Quality.