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Antibody / Mab Fab production Service

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Antibody Fab Production Service

Host Cells: HEK293 or CHO cell (please specify, cost for production in CHO cell is higher)
We provide antibody Fab production in E. coli. expression system as well,
If you prefer E. coli. system, please specify in your request.
Production: Recombinant technology, transient transfection, Serum-free cell culture
Purification: His-tag or FLAG-tag Affinity chromatography column purification
Protein A or G affinity purification maybe feasible for some Fabs without a His-tag/FLAG tag
Timeline: 5-8 weeks total from receiving gene sequence to antibody delivery
- 1-2 weeks for gene synthesis
- 1-2 weeks for vector construction and plasmid production
- 3-4 weeks for transient transfection, antibody purification, QC testing
Deliverable: Purified antibody Fab (quantities to be specified by customer)
Quality Specification: as specified by cutomer (cost may be adjusted based on quality spec)
Antibody Types: human, mouse, rat, rabbit, canine, rhesus IgG antibodies
Applications: drug discovery: antibody antigen co-crystalization, antibody binding eptiope
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas
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Features of Antibody Production Services at Sino Biological

  • Record Speed in Antibody Production
  • • From Mab Sequence to Purified
  • • 1-100 mg Mab in 3-6 weeks
  • • 1-10 gram Mab in 6-8 weeks
  • GLP Grade Antibody Production
  • • High Monomer Purity: up to 99%
  • • Low Endotoxin Level: up to 0.05 EU/mg
  • Unmatched Experience in Antibody Production
  • • >10,000 Mab Production
  • • Almost 100% success rate
Cutting-Edge Technology Unlimited Capacity Unbelievable Cost-Saving

Antibody Fab Production Service Description

Sino Biological's has developed transient transfection technology in HEK293 cells for antibody Fab expression and production, and has generated excellent quality of Fab fragment for antigen-Fab co-crystallization. The recombinant antibody Fab fragment protein produced by our transient transfection in mammalian cells is of superior quality than antibody fragment protein generated from enzyme cleavage of full antibodies. If customers prefer, we can also use our E. coli. expression system for antibody Fab production.

Because many antibody Fab fragment proteins do not bind well to protein A, protein G, or protein L affinity resin, we typically add a poly-histidine tag at the C-terminus of the antibody heave chain for purification. A protease cleavage site can be designed between the His-tag and the Fab to allow tag-removal after purification if needed.

You Pay on Deliverable, Risk-Free:

For customers that have set up a contract service account with us, we typically do not require an upfront payment ...... <Learn More>

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
- Antibody CRO Service Description
- Antibody Service Q&A
- Antibody /Mab Production CRO Service
-- Antibody Production Service Description
-- Ab Production Service Q&A
-- Antibody /Mab Production Service: mini-scale (0.1-1 mg)
mini-scale Mab production description
mini-scale Mab production Q&A
-- Antibody /Mab Production Service: Small-scale (1-10 mg)
small-scale Mab production description
small scale Mab production Q&A
-- Antibody /Mab Production Service: Mid-scale (10-100 mg)
Mid-scale Mab production Description
Mid-scale Mab production Q&A
-- Antibody /Mab Production Service: Large-scale (100 mg - 1 gram)
Large-scale Mab production Description
Large scale Mab production Q&A
-- Transient Transfection Antibody Production: Super-scale (1g - 100 gram)
Super-scale Mab by Transient transfection Description
Super-scale Mab by Transient transfection Q&A
-- Stable CHO Antibody Production Service: Super-scale (1g - 100 gram)
Super Scale Mab by CHO Description
-- Single-Chain ScFv Antibody Fragment Production Service
ScFv antibody fragment Description
-- Antibody / Mab Fab production Service
Antibody Fab Production Service Description
-- Mouse Mab production CRO Service
Mouse Mab Production Description
mouse Mab production from hybridoma cell culture
recombinant mouse Mab production service
-- Rat antibody production CRO service
Description of Rat Monoclonal Antibody Production Service
Rat Mab production from hybridoma cell culture
Recombinant Rat Mab production service
-- Rabbit Mab production CRO Service
Rabbit Mab production service Description
Rabbit Antibody /Mab Production Service: Hybridoma Culture
Recombinant Rabbit Mab Production Service
- Antibody Purification Service
- Antibody Humanization Service / Services
- Custom Antibody Development Service / Services
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"