Sino Biological has developed a technology platform in mammalian cells for the expression and production of antibody Fab fragments. The recombinant antibody Fab fragments produced by our technology is of superior quality than antibody Fab fragments generated from enzyme cleavage of full antibodies.
ScFv (single-chain Fragment variable) antibody expression and production is a routine work to support antibody discovery with antibody library. ScFv protein can be readily expressed in microbial expression system such as E. coli. It can also be expressed in mammalian cells through transient transfection. Sino Biological is capable of producing ScFv single chain antibody fragment using either production systems, depending on customer's preference.
|Gene synthesis and codon optimization (optional)||1-2 weeks||• Purified antibody
• SDS-PAGE data
|Vector construction||1-2 weeks|
|• Expression vector construction
• Plasmid sequencing
• Large scale plasmid production
|Expression & purification||1-2 weeks|
|• Transfection of HEK293 / CHO cells or E.coli. fermentation
• Purify the antibody
• Polishing if needed
|QC analysis||3 days|
|• Analysis by SDS-PAGE and UV
• Purity analysis by HPLC-SEC and endotoxin level analysis
• Client needs to provide the antibody sequence or plasmid.
• A standard service includes: antibody concentration detected by A280 and purity evaluated by SDS-PAGE. If other detection services such as SEC-HPLC and endotoxin test are required, please indicate it in the request form.