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Antibody Affinity Maturation Service

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Antibody Affinity Maturation Service

Service: human, mouse, rat, rabbit antibody affinity maturation
Technology: Random gene mutation, phage-display antibody library
Higher affinity antibody screening and detection
Affinity optimized antibody analysis and design
Recombinant antibody expression
Host Cell for Screening: E. coli. and Phage
Production Host: HEK293 or CHO cell transient transfection for antibody production
Timeline: 15~21 weeks from mouse or rabbit antibody DNA to mg of humanization antibody
2 weeks for antibody mutation library design
2-3 weeks for mutation antibody phage library
3-5 weeks for affinity optimized antibody screening and detection
2 weeks for antibody expression and affinity detection
1 week for antibody sequencing and mutation analysis
1 week for gene synthesis or mutation
1-2 weeks for vector construction and plasmid production
2-4 weeks for transient expression, antibody purification, QC testing
1 week for affinity analysisuire.
Deliverable: 4~16 affinity optimized antibody sequences
1 – 2 mg purified antibody for each molecule for testing
Larger quantity of antibody production can be requested
(please refer to our antibody production service).
Applications: therapeutic antibody discovery & development
Quotation: on-line request form or e-mail request to CRO-service@sinobiological.com

Track Records & Key Facts

  • • > 300 Staff
  • • > 80 Bioreactors
  • • > 30 Purification System
  • • > 10,000 Mab Production
  • • > 5000 Protein Bulks
  • • > 5000 antibody development
  • • 1-100mg Mab Production in 3-6 weeks
  • • 1-10 g Mab Production in 6-8 weeks
  • • Preferred CRO Partner for Multiple Top-10 Pharmas

Antibody Affinity Maturation Service Description

To achieve certain level of therapeutic effects in vivo, an antibody needs to reach a minimum affinity level. However, in some cases it may not be easy to screening for antibodies that possess the right biological function and have very high antigen binding affinity in the same time. In this case, improving an existing antibody's antigen-binding affinity might be the best approach one could take the antibody into clinical development. In some other cases, an antibody's affinity can drop significantly after humanization. Hence increase the humanized antibody's affinity back to the level before its humanization may be necessary. Affinity maturation is a common strategy to improve an antibody's antigen-binding affinity.

With its in-house phage-display technology and molecule biology techniques...... <Learn More>