Anti-CD68 Antibody

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Anti-CD68 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CD68 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD68
Immunogen
Recombinant Human CD68 Protein (Catalog#11192-H08B1)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD68 (rh CD68; Catalog#11192-H08B1; P34810-1; Met1-Ser319). CD68 specific IgG was purified by Human CD68 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD68 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:3000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD68 Antibody (Rabbit Polyclonal antibody) Images

Anti-CD68 rabbit polyclonal antibody at 1:1000 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:37 kDa

Observed band size:37 kDa

Immunochemical staining of human CD68 in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CD68 in human lung with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

Anti-CD68 Antibody: Alternative Names

Anti-GP110 Antibody; Anti-LAMP4 Antibody; Anti-SCARD1 Antibody

CD68 Background Information

Macrosialin, also known as CD68 and Gp11, is a single-pass type I membrane protein which belongs to theLAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Full Name
CD68 molecule
References
  • Strobl H. et al., 1995, Br J Haematol. 90 (4): 774-82.
  • Ogawa Y. et al., 1995, Pathol Int. 45 (9): 698-701.
  • Sadovnikova E. et al., 2002,Leukemia. 16 (10): 2019-26.
  • Gottfried E. et al., 2008, Scand J Immunol. 67 (5): 453-63.
  • Strojnik T. et al., 2009, Anticancer Res. 29 (8): 3269-79.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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