Anti-Angiopoietin-2 Antibody

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Anti-Angiopoietin-2 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Angiopoietin-2 Antibody
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Angiopoietin-2
Immunogen
Recombinant Human Angiopoietin-2 / ANG 2 protein (Catalog#10691-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Angiopoietin-2 / ANG 2 (rh Angiopoietin-2 / ANG 2; Catalog#10691-H08H; NP_001138.1; Met1-Phe496).
Source
Monoclonal Rabbit IgG Clone #117
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Angiopoietin-2 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:100-1:500
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Angiopoietin-2 Antibody (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ang2 monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

Anti-Ang2 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:70 kDa

Ang2 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

Lane C:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-Ang2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Ang2 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 57 kDa

Anti-Angiopoietin-2 Antibody: Alternative Names

Anti-AGPT2 Antibody; Anti-ANG2 Antibody; Anti-Angiopoietin-2 Antibody

Angiopoietin-2 Background Information

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
Full Name
angiopoietin 2
References
  • Thomas M, et al. (2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37.
  • Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
  • Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
  • Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.

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