Mouse GLA Gene cDNA Clone / ORF Clone

GLA cDNA Clone Datasheet

GLA cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013463.2
• cDNA Size: 1266
• Gene Synonym: Ags; Gla
• Source: Mouse

GLA cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus galactosidase, alpha DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 906 G/C not causing the amino acid variation.

GLA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GLA cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GLA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCATGA AGCTTTTGAG CAGAGATACG CGGCTGGTCT GTGAGCTTGC GCTTTGTCCC
61       CTGGCTTTAG TTTTCTGGAG CATTCTTGGG GTCAGAGCAT TGGACAATGG CTTGGCGCGG
121      ACTCCTACTA TGGGCTGGCT GCATTGGGAA CGTTTCATGT GCAACCTTGA CTGCCAAGAA
181      GAGCCTGATG CCTGCATAAG TGAGCAACTG TTCATGCAGA TGGCAGAGCT CATGGTCTCT
241      GATGGCTGGC GGGATGCAGG TTATGACTAT CTCTGCATAG ATGACTGTTG GATGGCTCCC
301      GAGAGGGATT CAAAGGGCAG GCTTCAGGCA GATCCCCAAC GCTTTCCTAG TGGGATCAAA
361      CACCTCGCAA ATTACGTCCA CAGCAAAGGA TTGAAGCTAG GGATTTATGC AGATGTTGGG
421      AATAAAACCT GTGCAGGTTT CCCCGGGAGT TTTGGATCCT ATGACATTGA TGCGCAGACA
481      TTTGCTGACT GGGGCGTAGA TCTGCTAAAA TTTGATGGTT GTCACTGTGA CAGTGTAGTA
541      TCCTTGGAGA ATGGTTATAA GTACATGGCC TTGGCCTTGA ACAGGACAGG CCGAAGCATT
601      GTATACTCCT GTGAGTGGCC ACTTTATTTG AGACCCTTTC ATAAGCCCAA TTATACAGAT
661      ATCCAATATT ACTGCAATCA TTGGAGAAAT TTTGATGATG TTTATGATTC CTGGGAAAGC
721      ATAAAGAATA TCTTGTCTTG GACAGTGGTT TACCAGAAGG AGATTGTTGA AGTCGCTGGA
781      CCAGGCAGCT GGAATGACCC AGACATGTTA GTGATCGGCA ACTTTGGCCT CAGTTGGGAC
841      CAGCAGGTGA CACAGATGGC CCTCTGGGCT ATCATGGCCG CTCCCCTACT CATGTCCAAC
901      GATCTCCGAC AAATCAGCTC TCAAGCCAAA GCTCTGCTTC AGAATAAGGA TGTAATTGCC
961      ATCAACCAAG ACCCCTTGGG CAAGCAGGGC TACTGTTTCA GAAAGGAAAA CCACATTGAG
1021     GTTTGGGAAC GGCCACTCTC CAACCTAGCC TGGGCTGTGG CTGTGAGAAA CCTGCAGGAG
1081     ATTGGTGGAC CTTGTCCTTA TACCATCCAG ATTTCTTCCC TGGGTAGAGG ACTAGCCTGC
1141     AATCCTGGCT GCATCATTAC TCAGCTTCTC CCCGAGAAAG TACACCTAGG CTTCTATGAA
1201     TGGACTTTGA CCTTAAAAAC TCGAGTAAAC CCCTCAGGCA CTGTTTTGTT TCGGTTAGAA
1261     AGATAA

 

Quality Control:cDNA sequencing results is available upon request.