Anti-GLA Antibody

Cat: 12078-RP02
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Anti-GLA Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GLA Antibody
Validated applications
Species reactivity
Reacts with: Human
Human GLA
Recombinant Human alpha-Galactosidase A / GLA protein (Catalog#12078-H08H)
Produced in rabbits immunized with purified, recombinant Human alpha-Galactosidase A (rh alpha-Galactosidase A / GLA; Catalog#12078-H08H; AAP36507.1; Met 1-Leu 429). alpha-Galactosidase A / GLA specific IgG was purified by Human alpha-Galactosidase A / GLA affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GLA Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GLA Antibody (Rabbit Polyclonal antibody) Images
Human alpha-Galactosidase A Immunohistochemistry(IHC) 4131
Immunochemical staining of human GLA in human prostatic carcinoma with rabbit polyclonal antibody(1:1000, formalin-fixed paraffin embedded sections).
Human alpha-Galactosidase A Immunohistochemistry(IHC) 4132
Immunochemical staining of human GLA in human breast carcinoma (from 2 donors) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human alpha-Galactosidase A Western blot (WB) 13466

Anti-GLA rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:49 kDa

Observed band size:49 kDa

Human alpha-Galactosidase A Immunoprecipitation(IP) 17698

GLA was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

2 µL anti-GLA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-GLA rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 49 kDa

Observed band size: 52 kDa

Anti-GLA Antibody Alternative Names
Anti-GALA Antibody
GLA Background Information

Alpha-galactosidase A, also known as Alpha-D-galactoside galactohydrolase, Alpha-D-galactosidase A, Melibiase and GLA, is a member of the glycosyl hydrolase 27 family. GLA is used as a long-term enzyme replacement therapy in patients with a confirmed diagnosis of Fabry disease. Defects in GLA are the cause of Fabry disease (FD) which is a rare X-linked sphingolipidosis disease where glycolipid accumulates in many tissues. The disease consists of an inborn error of glycosphingolipid catabolism. FD patients show systemic accumulation of globotriaoslyceramide (Gb3) and related glycosphingolipids in the plasma and cellular lysosomes throughout the body. Clinical recognition in males results from characteristic skin lesions (angiokeratomas) over the lower trunk. Patients may show ocular deposits, febrile episodes, and burning pain in the extremities. Death results from renal failure, cardiac or cerebral complications of hypertension or other vascular disease. Deficiency of GLA leads to the accumulation of glycosphingolipids in the vasculature leading to multiorgan pathology. In addition to well-described microvascular disease, deficiency of GLA is also characterized by premature macrovascular events such as stroke and possibly myocardial infarction.

Full Name
galactosidase, alpha
  • Koide al., 1990, FEBS Lett. 259:353-356.
  • Yang C.-C. et al., 2003, Clin. Genet. 63:205-209.
  • Verovnik F. et al.,2004, Eur. J. Hum. Genet. 12:678-681.
  • Nance C.S. et al., 2006, Arch. Neurol. 63:453-457.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GLA Antibody Rabbit Polyclonal ELISA 50964-RP01
    Anti-GLA Antibody Rabbit Polyclonal ELISA 50964-T16
    Anti-GLA Antibody Rabbit Polyclonal ELISA,IP 12078-T20
    Anti-GLA Antibody Mouse Monoclonal ELISA,ELISA(Cap) 12078-MM08
    Anti-GLA Antibody Rabbit Polyclonal ELISA 12078-T16
    Anti-GLA Antibody Rabbit Polyclonal WB,ELISA,IP 12078-T52
    Anti-GLA Antibody Mouse Monoclonal ELISA 12078-MM07
    Anti-GLA Antibody Mouse Monoclonal ELISA(Det) 12078-MM05
    Anti-GLA Antibody Rabbit Monoclonal WB,ELISA,IP 12078-R001
    Anti-GLA Antibody Rabbit Monoclonal WB 12078-R201
    Anti-GLA Antibody Rabbit Polyclonal ELISA 12078-RP01
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