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acnB Antibody, Rabbit MAb

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acnBAntibody Product Information
Immunogen:Recombinant E. coli acnB protein (Catalog#13102-ENAE)
Clone ID:102
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant E. coli acnB (rE. coli acnB; Catalog#13102-ENAE; P36683; Met 1-Val 865).
acnBAntibody Usage Guide
Specificity:E. coli acnB
Application:ELISA

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect E. coli ACNB. The detection limit for E. coli ACNB is approximately 0.0049 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.

References
  • Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
  • Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
  • Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
  • Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.
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