Mouse aFGF / FGF1 Protein (Native)

Fibroblast Growth Factor 1 ( FGF1 / aFGF ) Protein

FGF1 / aFGF Price Inquiry ( Available Sizes )

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FGF1 / aFGF Protein Product Information

• Synonym: Dffrx, Fam, Fgf-1, Fgfa, Fgf1
• Protein Construction: A DNA sequence encoding the mature form of mouse FGF1 ( NP_034327.1 ) ( Phe 16 - Asp 155 ) was expressed.
• Source: Mouse
• Expression Host: E.Coli

FGF1 / aFGF Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.
  The ED₅₀ for this effect is typically 0.05-0.25 ng/mL in the presence of 10 μg/mL heparin
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse FGF1 consisting of 141 amino acids and has a calculated molecular mass of 16 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Na₂SO₄, 0.5mM DTT,1mM EDTA,20mM Tris, pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: aFGF protein

FGF1 / aFGF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF1 / aFGF Protein Description

Acidic fibroblast growth factor (aFGF), also known as heparin-binding growth factor (HBGF-1) and FGF1, is a member of FGF family. FGF family members are involved in a variety of biological processes. FGF1 is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis. This protein acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro and stimulates cell growth, migration, and differentiation. FGF1 binds to cell surface receptors with high affinity with the prerequisite association of FGF-acidic with heparan sulfate. The ligation subsequently initiates receptor dimerization, transphosphorylation, as well as internalization of receptor/FGF complexes, and thus aFGF is translocated across cellular membranes and transported to the nucleus. It has been demonstrated that PI 3-kinase activity is required both for transport of aFGF to the cytosol and to the nuclear fraction.

References

1. Wang, W.P. et al., 1991, Oncogene. 6: 1521-1529.
2. Engelmann, G.L. et al., 1993, Circ. Res. 72: 7-19.
3. Wiedlocha, A. et al., 1994, Cell. 76: 1039-1051.
4. Wiedlocha, A. et al., 1996, Mol. Cell. Biol. 16: 270-280.
5. Munoz, R. et al., 1997, Oncogene. 15: 525-536.
6. Klingenberg, O. et al., 2000, J. Biol. Chem. 275: 11972-11980