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Human aFGF/FGF1 Gene ORF cDNA clone in cloning vector

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    Human aFGF/FGF1 cDNA Clone Product Information
    NCBI RefSeq:
    RefSeq ORF Size:468bp
    cDNA Description:Full length Clone DNA of Homo sapiens fibroblast growth factor 1 (acidic).
    Gene Synonym:FGF1, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
    Species:Human
    Vector:pMD18-T Simple Vector
    Plasmid:pMD-aFGF
    Restriction Site:
    Tag Sequence:
    Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in E. coli system. The translated amino acid sequence is identical with NP_000791.1.
    Sequencing primers:M13-47 and RV-M
    ( We provide with FGF1 qPCR primers for gene expression analysis, HP100110 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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    Background

    aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

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    Catalog: HG10013-M
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"