FGF1 cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Sequence Description
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in E. coli system. The translated amino acid sequence is identical with NP_000791.1.
Description
Full length Clone DNA of Human fibroblast growth factor 1 (acidic) with Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Restriction Sites
KpnI + XhoI (5.5kb + 0.5kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
FGF1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
FGF1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
AFGF cDNA ORF Clone, Human;ECGF cDNA ORF Clone, Human;ECGF-beta cDNA ORF Clone, Human;ECGFA cDNA ORF Clone, Human;ECGFB cDNA ORF Clone, Human;FGF-1 cDNA ORF Clone, Human;FGF-alpha cDNA ORF Clone, Human;FGFA cDNA ORF Clone, Human;GLIO703 cDNA ORF Clone, Human;HBGF-1 cDNA ORF Clone, Human;HBGF1 cDNA ORF Clone, Human
FGF1 Background Information
aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.
References
Full Name
fibroblast growth factor 1 (acidic)
References
Jaye M., et al.,(1986), Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization. Science 233:541-545.Mergia A., et al., (1989), Structural analysis of the gene for human acidic fibroblast growth factor.Biochem. Biophys. Res. Commun. 164:1121-1129.Wang W.P., et al.,(1989), Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues.Mol. Cell. Biol. 9:2387-2395.
