FGF1 / aFGF Antibody

Mouse Acidic Fibroblast Growth Factor ( FGF1 / aFGF ) Antibody

FGF1 / aFGF Antibody Product Information

• Immunogen: Recombinant mouse aFGF protein ( Catalog#50179-MNAE )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, E.coli-derived, recombinant mouse aFGF ( rM aFGF ; Catalog#50179-MNAE ; NP_034327.1 ; Phe 16 - Asp 155 ). Total IgG was purified by Protein A affinity chromatography.

FGF1 / aFGF Antibody Usage Guide

• Specificity: Mouse FGF1 / aFGF
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse FGF1 in WB
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect mouse FGF1. The detection limit for mouse FGF1 is 0.039 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FGF1 / aFGF Antibody Background

Mouse acidic fibroblast growth factor (aFGF), also known as heparin-binding growth factor (HBGF-1) and FGF1, is a member of FGF family. FGF family members are involved in a variety of biological processes. FGF1 is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis. This protein acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro and stimulates cell growth, migration, and differentiation. FGF1 binds to cell surface receptors with high affinity with the prerequisite association of FGF-acidic with heparan sulfate. The ligation subsequently initiates receptor dimerization, transphosphorylation, as well as internalization of receptor/FGF complexes, and thus aFGF is translocated across cellular membranes and transported to the nucleus. It has been demonstrated that PI 3-kinase activity is required both for transport of aFGF to the cytosol and to the nuclear fraction.

References

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