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Human ZPBP Gene ORF cDNA clone in cloning vector

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Human ZPBP cDNA Clone Product Information
NCBI RefSeq:BC005223
RefSeq ORF Size:1053
cDNA Description:ORF Clone of Homo sapiens zona pellucida binding protein DNA.
Gene Synonym:ZPBP1, ZPBP
Species:Human
Vector:pGEM-T Vector
Plasmid:
Restriction Site:
Tag Sequence:
Sequence Description:Same as Gene Bank Ref. ID sequence with the nucleotide 1054-1056 TAA (Stop codon) deletion mutation.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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