Human ZPBP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13288-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ZPBP cDNA Clone Datasheet

ZPBP cDNA Clone Product Information

Gene Bank Ref.ID:	BC005223
cDNA Size:	1053
Gene Synonym:	ZPBP1; ZPBP
Source:	Human

ZPBP cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens zona pellucida binding protein DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 1054-1056 TAA deletion mutation.

ZPBP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ZPBP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ZPBP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGGCCT TCGCCCTTGG CCCAGCGCGG CGGGGCAGGC GGCGGACCCG GGCCGCCGGC
61       TCCCTGCTCT CTCGGGCCGC CATCCTCCTC TTTATCTCCG CCTTCCTGGT GCGGGTGCCC
121      TCATCAGTTG GACACTTGGT TCGATTACCA AGAGCTTTTC GCTTGACCAA AGATTCAGTG
181      AAAATAGTGG GATCAACAAG TTTTCCAGTG AAAGCGTATG TCATGCTCCA TCAAAAGAGT
241      CCACACGTGT TATGTGTAAC GCAACAACTG CGAAATGCTG AACTGATAGA CCCATCATTC
301      CAATGGTATG GGCCTAAAGG AAAAGTTGTT TCAGTAGAAA ACCGCACTGC ACAAATAACA
361      TCCACAGGAA GCCTTGTATT CCAAAATTTT GAGGAGAGTA TGAGTGGAAT TTATACATGT
421      TTCCTCGAAT ATAAACCTAC TGTGGAAGAA ATTGTTAAAC GTCTTCAACT AAAATATGCT
481      ATATATGCTT ATCGTGAGCC TCATTATTAT TATCAGTTCA CAGCTCGATA TCATGCAGCT
541      CCCTGCAATA GCATTTATAA TATTTCTTTT GAGAAGAAAC TTCTTCAGAT TTTAAGCAAA
601      CTGCTTCTTG ACCTTTCATG TGAAATTTCC TTACTTAAGT CTGAATGCCA TCGCGTTAAA
661      ATGCAAAGAG CTGGTTTGCA AAATGAATTG TTCTTTGCAT TTTCAGTTTC ATCTCTAGAC
721      ACTGAAAAAG GACCCAAGCG ATGTACAGAC CATAACTGTG AACCTTACAA AAGACTTTTT
781      AAGGCTAAAA ATCTCATAGA GAGATTTTTT AATCAACAAG TAGAAATTCT TGGCAGACGT
841      GCAGAACAAT TACCTCAAAT ATACTATATT GAAGGTACTC TCCAAATGGT TTGGATTAAT
901      CGCTGCTTTC CAGGATATGG AATGAATGTC CAGCAACATC CAAAATGTCC TGAGTGCTGT
961      GTGATCTGCA GCCCTGGATC ATATAACCCC CGTGATGGAA TTCATTGCCT TCAATGCAAT
1021     AGCAGCCTGG TGTATGGAGC AAAAACGTGC TTA

Quality Control:cDNA sequencing results is available upon request.