Mouse ZBTB16 Gene cDNA Clone / ORF Clone

ZBTB16 cDNA Clone Datasheet

ZBTB16 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033324.2
• cDNA Size: 2022
• Gene Synonym: lu; PLZF; Zfp145; AI467657; Zbtb16
• Source: Mouse

ZBTB16 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus zinc finger and BTB domain containing 16 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 216 A/G,606 C/T,861 T/C,1062 C/T,1221 C/T,1395 A/G,1398 C/T and 1731 T/C not causing the amino acid variation.

ZBTB16 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ZBTB16 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ZBTB16 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCTGA CAAAGATGGG GATGATCCAG CTGCAGAACC CTAGCCACCC CACGGGGCTG
61       CTGTGCAAGG CCAATCAGAT GCGACTGGCT GGGACTTTGT GCGATGTGGT CATCATGGTG
121      GACAGCCAGG AGTTCCATGC CCACCGGACG GTGCTAGCCT GCACCAGCAA GATGTTTGAG
181      ATCCTCTTCC ACCGAAACAG CCAGCACTAT ACTCTGGACT TCCTCTCGCC AAAAACCTTC
241      CAGCAGATCC TGGAGTACGC CTACACGGCC ACACTGCAAG CCAAGGCGGA GGACCTGGAT
301      GACCTGCTGT ATGCAGCTGA GATTTTAGAG ATCGAATACC TGGAGGAGCA GTGCCTGAAG
361      ATCCTGGAGA CCATCCAGGC ATCTGATGAC AATGACACAG AGGCCACCAT GGCTGACGGT
421      GGGGGCGAAG AAGAAGAGGA CCGTAAGGCT CGATACCTCA AGAACATCTT TATCTCGAAG
481      CATTCCAGCG AGGAGAGTGG CTACGCCAGT GTGGCTGGAC AGAGCCTCCC TGGGCCCATG
541      GTGGACCAGA GCCCCTCAGT CTCCACCTCT TTCGGTCTCT CAGCCATGAG TCCTACCAAG
601      GCAGCTGTGG ACAGCTTGAT GAGTATAGGA CAGTCACTCC TGCAAGGAAC TCTTCAGCCA
661      CCTGCAGGGC CTGAGGAGCC CACACTGGCA GGGGGTGGGC GACACCCTGG GGTGGCTGAG
721      GTGAAGATGG AAATGATGCA GGTAGATGAA GCCCCTGGCC AGGACAGCCC TGGGGCAGCT
781      GAGTCTAGCA TCTCAGGAGG GATGGGGGAC AAGCTTGAAG AGAGGAGCAA AGAGGGGCCT
841      GGGACCCCGA CTCGAGGCAG CGTCATCACT AGTGCCAGAG AGCTGCATTA TGGGAGAGAG
901      GAGAGTGGTG AGCAGCTCTC GCCTCCTGTT GAAGCTGGCC AGGGACCCCC TGGGCGGCAG
961      GAGCCCCTGG CACCCCCAGT GGAGAAGCAT TTGGGTATCT ACTCGGTGCT GCCCAACCAC
1021     AAGGCCGATG CTGTGTTGAG CATGCCGTCT TCAGTGACGT CTGGTCTCCA TGTGCAACCT
1081     GCCCTGGCAG TCTCCATGGA CTTCAGCACC TACGGGGGTC TGCTGCCTCA GGGCTTCATC
1141     CAGAGGGAGC TGTTCAGCAA GCTGGGGGAG CTGGCTGTGG GCATGAAGGC TGAGAGCCGC
1201     CCTCTGGGGG AGCAGTGCAG TGTGTGTGGG GTCGAGCTTC CGGACAACGA GGCAGTGGAG
1261     CAGCACAGGA AACTGCACAG TGGGATGAAA ACATACGGGT GTGAACTCTG CGGAAAACGG
1321     TTCCTGGACA GTTTGCGACT GAGAATGCAT TTACTGGCTC ATTCAGCGGG TGCCAAAGCC
1381     TTTGTGTGTG ATCAGTGTGG TGCCCAGTTC TCAAAGGAGG ATGCCCTGGA GACACACAGA
1441     CAGACCCATA CTGGCACGGA CATGGCTGTC TTCTGTCTGC TGTGTGGGAA ACGCTTTCAG
1501     GCACAAAGCG CACTCCAGCA GCACATGGAG GTCCACGCAG GCGTGCGCAG CTATATTTGC
1561     AGTGAGTGCA ACCGCACCTT CCCCAGCCAC ACGGCTCTCA AGCGCCACCT TCGCTCACAT
1621     ACAGGTGACC ACCCATATGA GTGTGAGTTC TGCGGCAGCT GCTTCCGGGA TGAGAGCACA
1681     CTCAAGAGCC ACAAGCGCAT CCACACAGGG GAGAAGCCCT ATGAGTGTAA CGGCTGTGGC
1741     AAGAAGTTCA GCCTCAAGCA CCAGTTGGAG ACGCACTACA GGGTTCACAC AGGTGAGAAG
1801     CCCTTTGAGT GCAAACTCTG CCACCAGCGC TCCCGAGACT ACTCGGCCAT GATCAAGCAC
1861     CTGAGAACAC ACAACGGTGC CTCGCCATAC CAGTGTACCA TCTGCACGGA ATACTGCCCC
1921     AGCCTCTCCT CCATGCAGAA ACACATGAAG GGCCACAAGC CGGAGGAGAT CCCACCTGAC
1981     TGGAGGATAG AGAAGACGTA CCTCTACCTG TGTTATGTGT GA

 

Quality Control:cDNA sequencing results is available upon request.