Cynomolgus XCL1 Protein (His Tag) (Cytokine)

Chemokine (C motif) ligand 1 Protein Datasheet

XCL1 Protein Product Information

• Synonym: XCL1
• Protein Construction: A DNA sequence encoding the Cynomolgus (Macaca fascicularis) XCL1 (Met 1- Gly 114) was fused with a polyhistidine tag at the C-terminus.
• Source: Cynomolgus
• Expression Host: Baculovirus-Insect cells

XCL1 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 22
• Molecular Mass: The secreted recombinant cynomolgus XCL1 consists of 103 amino acids and has a calculated molecular mass of 11.6 kDa. The apparent molecular mass of the recombinant protein is approximately 18-23 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 20mM Tris 500mM NaCl, PH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: XCL1 protein

XCL1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

XCL1 Protein Description

Chemokine (C motif) ligand (XCL1) is a member of the C-chemokine subfamily of XC chemokine family that is also known as lymphotactin. Chemokines are the largest family of cytokines, cause leukocyte mobilization through chemotaxis at sites of antigenic challenge or lesions. XCL1 is maily expressed in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and lower levels in lung, prostate gland and ovary. XCL1 differs from other chemokines in that it contains only a single disulfide bond and a mucin-like domain at its carboxy terminus that is glycosylated. Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. This chemokine attracts T cells. Experiments showed a reduction in XCL1 and XCR1 expression in allergic asthma regulatory T cell compared with healthy control and nonallergic asthmatic counterparts. This reduction in XCL1 expression was associated with the suboptimal regulatory function of regulatory T cell (Treg) in allergic asthma. Interestingly, incubation with recombinant human XCL1 significantly increased Treg-mediated suppression and cytotoxicity by up-regulating expression of XCL1 and chief effector molecules of Treg function. Altogether, these results suggest an association between dysregulated XCL1 expression and reduced Treg activities in AA, as well as a potential role of XCL1 in reversing defective Treg function in the disease.

References

1. Nguyen KD. et al., 2008, J Immunol. 181 (8): 5386-95.
2. Dong C. et al., 2005, J Immunol Methods. 302 (1-2): 136-44.
3. Zavala-Flores LM. et al., 2009, Biotechnol Lett. 31 (2): 215-20.