Wnt Receptor

Sino Biological offers a wide selection of tools for studying Wnt signaling pathways, including the study of Wnt ligands, receptors, and inhibitors, canonical Wnt pathway and non-canonical Wnt pathways. These quality reagents include recombinant proteins, antibodies, ELISA kits, and ORF cDNA clones.

Wnt Receptor Products

Frizzled Family

• Frizzled-1 / FZD1
• Frizzled-4 / FZD4
• Frizzled-10 / FZD10
• Frizzled-2 / FZD2
• Frizzled-5 / FZD5

Wnt Receptor Background

Reception and transduction of Wnt signals involves binding of Wnt proteins to members of two distinct families of cellsurface receptors, members of the Frizzled gene family and members of the LDL-receptor-related protein (LRP) family. The canonical Frizzled receptor has an aminoterminal cysteine-rich domain (CRD) that binds Wnt, seven transmembrane domains and a short cytoplasmic tail containing a consensus PDZ domain binding motif at the carboxyl terminus. The CRD forms a novel protein fold with a conserved dimerization interface that may be important for Wnt binding. Frizzled receptors have been identified in vertebrates and invertebrates; there are ten known members in humans and mice, four in flies, and three in worms. The general structure of Frizzled receptors resembles that of seven-transmembrane G-protein-coupled receptors, suggesting that Frizzled proteins may use heterotrimeric G proteins to transduce Wnt signals. Several recent studies provide evidence consistent with this idea, showing that a subgroup of Frizzled receptors can signal through the pertussis-toxin-sensitive subclass of heterotrimeric G proteins to stimulate an increase in intracellular Ca²⁺ and activate protein kinase C (PKC). Heterotrimeric G proteins do not appear to be involved in transducing Wnt/Frizzled signals that regulate the cytoskeleton associated protein.

Wnt Receptor Related Studies

1. Miller JR. (2001) The Wnts. Protein family review. 3(1): 1-15.
2. Sheldahi LC, et al. (1999) Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner. Current Biology. 9(13): 695-8.
3. Liu X, et al. (1999) Activation of a frizzled-2/beta-adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via G_o and G_t2. PNAS. 96(25): 14383-8.
4. Wang HY, et al. (2006) Structure-function analysis of Frizzleds. Cellular Signalling. 18(7): 934-941.