All WTAP reagents are produced in house and quality controlled, including 2 WTAP Antibody, 29 WTAP Gene, 1 WTAP Protein, 2 WTAP qPCR. All WTAP reagents are ready to use.
Recombinant WTAP proteins are expressed by E. coli with fusion tags as N-GST.
WTAPantibodies are validated with different applications, which are WB, ELISA.
WTAPcDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each WTAP of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.