Human WT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12282-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

WT1 cDNA Clone Datasheet

WT1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC032861
cDNA Size:	909
Gene Synonym:	GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; WT1
Source:	Human

WT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens Wilms tumor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

WT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

WT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

WT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC
61       TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
121      CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
181      CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
241      AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
301      GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG
361      AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC
421      CAATACAGAA TGCACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG GCGTGTGCCT
481      GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG
541      TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC
601      AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT
661      TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG
721      TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT
781      CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG
841      TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG
901      GCGCTTTGA

Quality Control:cDNA sequencing results is available upon request.