Human WNT5B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12157-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

WNT5B cDNA Clone Datasheet

WNT5B cDNA Clone Product Information

Gene Bank Ref.ID:	BC001749
cDNA Size:	1080
Gene Synonym:	MGC2648; WNT5B
Source:	Human

WNT5B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens wingless-type MMTV integration site family, member DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

WNT5B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

WNT5B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

WNT5B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCAGCC TGCTGCTGCT GTTCACGGCT GCTCTGCTGT CCAGCTGGGC TCAGCTTCTG
61       ACAGACGCCA ACTCCTGGTG GTCATTAGCT TTGAACCCGG TGCAGAGACC CGAGATGTTT
121      ATCATCGGTG CCCAGCCCGT GTGCAGTCAG CTTCCCGGGC TCTCCCCTGG CCAGAGGAAG
181      CTGTGCCAAT TGTACCAGGA GCACATGGCC TACATAGGGG AGGGAGCCAA GACTGGCATC
241      AAGGAATGCC AGCACCAGTT CCGGCAGCGG CGGTGGAATT GCAGCACAGC GGACAACGCA
301      TCTGTCTTTG GGAGAGTCAT GCAGATAGGC AGCCGAGAGA CCGCCTTCAC CCACGCGGTG
361      AGCGCCGCGG GCGTGGTCAA CGCCATCAGC CGGGCCTGCC GCGAGGGCGA GCTCTCCACC
421      TGCGGCTGCA GCCGGACGGC GCGGCCCAAG GACCTGCCCC GGGACTGGCT GTGGGGCGGC
481      TGTGGGGACA ACGTGGAGTA CGGCTACCGC TTCGCCAAGG AGTTTGTGGA TGCCCGGGAG
541      CGAGAGAAGA ACTTTGCCAA AGGATCAGAG GAGCAGGGCC GGGTGCTCAT GAACCTGCAA
601      AACAACGAGG CCGGTCGCAG GGCTGTGTAT AAGATGGCAG ACGTAGCCTG CAAATGCCAC
661      GGCGTCTCGG GGTCCTGCAG CCTCAAGACC TGCTGGCTGC AGCTGGCCGA GTTCCGCAAG
721      GTCGGGGACC GGCTGAAGGA GAAGTACGAC AGCGCGGCCG CCATGCGCGT CACCCGCAAG
781      GGCCGGCTGG AGCTGGTCAA CAGCCGCTTC ACCCAGCCCA CCCCGGAGGA CCTGGTCTAT
841      GTGGACCCCA GCCCCGACTA CTGCCTGCGC AACGAGAGCA CGGGCTCCCT GGGCACGCAG
901      GGCCGCCTCT GCAACAAGAC CTCGGAGGGC ATGGATGGCT GTGAGCTCAT GTGCTGCGGG
961      CGTGGCTACA ACCAGTTCAA GAGCGTGCAG GTGGAGCGCT GCCACTGCAA GTTCCACTGG
1021     TGCTGCTTCG TCAGGTGTAA GAAGTGCACG GAGATCGTGG ACCAGTACAT CTGTAAATAG

Quality Control:cDNA sequencing results is available upon request.