Mouse WIF1 Gene cDNA Clone / ORF Clone

WIF1 cDNA Clone Datasheet

WIF1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011915.2
• cDNA Size: 1140
• Gene Synonym: WIF-1; AW107799; Wif1
• Source: Mouse

WIF1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Wnt inhibitory factor 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

WIF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

WIF1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

WIF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCGGA GAAGAGCCTT CCCTGCTTTC GCGCTCCGGC TCTGGAGCAT CCTACCTTGC
61       CTGCTCCTGC TGCGAGCGGA TGCAGGGCAG CCACCTGAGG AGAGCTTGTA CCTGTGGATC
121      GACGCCCATC AGGCTAGAGT GCTCATAGGA TTTGAAGAAG ACATTCTGAT TGTCTCGGAG
181      GGGAAAATGG CCCCCTTTAC ACATGATTTC AGGAAAGCCC AACAAAGAAT GCCAGCCATT
241      CCTGTCAATA TCCACTCCAT GAATTTTACC TGGCAAGCTG CGGGGCAGGC AGAATACTTC
301      TACGAGTTCC TGTCTCTGCG CTCCCTGGAT AAAGGCATCA TGGCAGATCC AACTGTCAAT
361      GTCCCTTTGC TGGGAACAGT GCCTCACAAG GCATCAGTTG TTCAAGTTGG TTTCCCGTGT
421      CTCGGCAAAC AAGACGGGGT AGCAGCATTT GAAGTGAATG TGATTGTCAT GAATTCTGAA
481      GGCAACACCA TCCTTAGGAC CCCTCAGAAT GCCATCTTCT TTAAAACATG TCAACAAGCT
541      GAGTGTCCCG GAGGGTGTCG AAATGGAGGC TTTTGTAACG AAAGGCGGGT CTGCGAGTGT
601      CCGGATGGGT TCTACGGGCC TCACTGTGAG AAAGCCCTGT GCATACCCCG ATGTATGAAC
661      GGTGGTCTGT GTGTCACTCC TGGCTTCTGC ATCTGCCCCC CTGGATTCTA CGGTGTCAAC
721      TGTGACAAAG CAAACTGCTC AACCACCTGC TTTAATGGAG GGACCTGCTT TTACCCGGGA
781      AAATGTATTT GCCCTCCTGG ACTCGAGGGA GAGCAGTGTG AACTCAGCAA ATGCCCCCAA
841      CCCTGCCGAA ATGGAGGTAA ATGCATTGGT AAAAGCAAGT GTAAGTGCCC GAAAGGTTAC
901      CAAGGAGACC TGTGCTCTAA GCCCGTCTGC GAGCCTGGCT GTGGTGCCCA CGGAACCTGC
961      CACGAACCCA ACAAGTGCCA GTGTCGAGAG GGCTGGCACG GCAGACACTG CAATAAGAGG
1021     TATGGAGCCA GCCTCATGCA TGCCCCGAGG CCAGCAGGCG CCGGGCTGGA GCGACACACG
1081     CCTTCACTTA AAAAGGCTGA GGATAGAAGG GATCCACCTG AATCCAATTA CATCTGGTGA

 

Quality Control:cDNA sequencing results is available upon request.