HE4 Protein, Human, Recombinant (His Tag) Product Information
> 95 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
Testing in progress
A DNA sequence encoding the human WFDC2 (NP_006094.3) (Met 1-Phe 124) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal
The secreted recombinant human WFDC2 comprises 105 amino acids with a predicted molecular mass of 11.3 kDa. As a result of glycosylation, the apparent molecular mass of rhWFDC2 is approximately 23-27 kDa in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us
for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
HE4 Protein, Human, Recombinant (His Tag) in SDS-PAGE
HE4 Protein, Human, Recombinant (His Tag) Alternative Names
dJ461P17.6 Protein, Human;EDDM4 Protein, Human;HE4 Protein, Human;WAP5 Protein, Human
HE4 Background Information
WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein which contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.
WAP four-disulfide core domain 2
Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73. Hellström,I. et al., 2003, Cancer Res. 63 (13):3695-700. Bingle,L. et al., 2006, Respir Res. 7 : 61. Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53. Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.