WFDC2 / HE4 Antibody (Antigen Affinity Purified)

WFDC2 / HE4 Antibody (Antigen Affinity Purified) Datasheet

WFDC2 / HE4 Antibody Product Information

• Immunogen: Recombinant Human WFDC2 / HE4 protein (Catalog #12609-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human WFDC2 / HE4 (rh WFDC2 / HE4; Catalog #12609-H08H; NP_006094.3; Met 1-Phe 124). WFDC2 / HE4 specific IgG was purified by Human WFDC2 / HE4 affinity chromatography.

WFDC2 / HE4 Antibody Usage Guide

• Specificity: Human WFDC2 / HE4
• Immunochemistry: IHC-P: 0.2-2 μg/mL

  

  Immunochemical staining of human WFDC2 in human endometrium with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to the epithelium of uterune gland.

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human WFDC2 in WB. Using a DAB detection system, the detection limit for Human WFDC2 is approximately 2 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect WFDC2. The detection limit for WFDC2 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

WFDC2 / HE4 Antibody Background

WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein which contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.

References

1. Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73.
2. Hellström,I. et al., 2003, Cancer Res. 63 (13):3695-700.
3. Bingle,L. et al., 2006, Respir Res. 7 : 61.
4. Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53.
5. Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.