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Human vinculin transcript variant 2 Gene cDNA Clone (full-length ORF Clone)
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Human vinculin transcript variant 2 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10019-M




  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_003373.3

cDNA Size:  3201

Synonym:   VCL, MVCL, CMD1W, Vin

Source:  Human.

cDNA Description:   ORF Clone of Homo sapiens vinculin (VCL), transcript variant 2 DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC
61       TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
121      ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
181      ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
241      GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
301      TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
361      GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
421      ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
481      ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
541      GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
601      TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
661      CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
721      ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
781      AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
841      AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
901      CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
961      ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
1021     AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
1081     GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
1141     AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
1201     GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
1261     TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
1321     CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
1381     GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
1441     GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
1501     CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
1561     GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
1621     CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
1681     AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
1741     AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
1801     GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
1861     AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
1921     GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
1981     ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
2041     ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
2101     CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
2161     TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
2221     ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
2281     AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
2341     GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
2401     GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
2461     GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
2521     GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
2581     CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
2641     AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
2701     GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT
2761     GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC
2821     AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG
2881     GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC
2941     CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA
3001     GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA
3061     GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA
3121     GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA
3181     AAGACTCCCT GGTACCAGTA G


Quality Control:   cDNA sequencing results is available upon request.

 

VCL/Vinculin related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"