pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication. Most commercially available competent cells are appropriate for the plasmid, e.g. TOP10, DH5α and TOP10F´, JM109. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene.
Usage Suggestion：The ORF cDNA sequence can be amplified by PCR with M13-47 and RV-M primers.