VWC2 Antibody (Antigen Affinity Purified)

VWC2 Antibody (Antigen Affinity Purified) Datasheet

VWC2 Antibody Product Information

• Immunogen: Recombinant Human VWC2 protein (Catalog #12071-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human VWC2 (rh VWC2; Catalog #12071-H08H; NP_055747.1; Met 1 - Met 325). VWC2 specific IgG was purified by Human VWC2 affinity chromatography.

VWC2 Antibody Usage Guide

• Specificity: Human VWC2
• Immunochemistry: IHC-P: 0.5-2 μg/mL

  

  Immunochemical staining of human VWC2 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human VWC2 in WB. Using a DAB detection system, the detection limit for Human VWC2 is approximately 0.5 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human VWC2. The detection limit for Human VWC2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

VWC2 Antibody Background

Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

References

1. Koike,N. et al., 2007, J Biol Chem. 282 (21):15843-50.
2. Elis,S. et al., 2009,Mol Reprod Dev. 76 (11):1043-55.
3. Zhang,J.L. et al., 2010,PLoS One. 5 (9):e12846.