VWC2 Antibody

VWC2 Antibody Datasheet

VWC2 Antibody Product Information

• Immunogen: Recombinant Human VWC2 Protein (Catalog #12071-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 10A5B4
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human VWC2 (rh VWC2; Catalog #12071-H08H; NP_055747.1; Met 1 - Met 325). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

VWC2 Antibody Usage Guide

• Specificity: Human VWC2
• No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human VWC2 in WB. Using a DAB detection system, the detection limit for Human VWC2 is approximately 4 ng/lane under non-reducing conditions and 50 ng/lane under reducing conditions.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

VWC2 Antibody Background

Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

References

1. Koike,N. et al., 2007, J Biol Chem. 282 (21):15843-50.
2. Elis,S. et al., 2009,Mol Reprod Dev. 76 (11):1043-55.
3. Zhang,J.L. et al., 2010,PLoS One. 5 (9):e12846.