VNN2 / Vanin-2 Antibody

VNN2 / Vanin-2 Antibody Datasheet

VNN2 / Vanin-2 Antibody Product Information

• Immunogen: Recombinant Human VNN2 protein (Catalog#11728-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3F8F8H6
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human VNN2 / Vanin-2 (rh VNN2 / Vanin-2; Catalog#11728-H08H; NP_004656.2; Met 1-Ser 492). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

VNN2 / Vanin-2 Antibody Usage Guide

• Specificity: Human VNN2 / Vanin-2
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human VNN2 in WB. Using a DAB detection system, the detection limit for Human VNN2 is approximately 1 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human VNN2. The detection limit for Human VNN2 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

VNN2 / Vanin-2 Antibody Background

Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-80, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.

References

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3. Liu T.et al., 2005, J. Proteome Res. 4: 2070-80.
4. Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.