Anti-VNN2 Antibody

Cat: 11728-MM04
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Anti-VNN2 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-VNN2 Antibody
Validated applications
ELISA,ELISA(Cap),FCM
Species reactivity
Reacts with: Human
Specificity
Human VNN2
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human VNN2 protein (Catalog#11728-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human VNN2 / Vanin-2 (rh VNN2 / Vanin-2; Catalog#11728-H08H; NP_004656.2; Met 1-Ser 492). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3F8F8H6
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-VNN2 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human VNN2 / Vanin-2 in ELISA pair set (Catalog: # SEK11728). In a sandwich ELISA, it can be used as capture antibody.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-VNN2 Antibody (Mouse Monoclonal antibody) Images
Human VNN2/Vanin-2 Flow Cytometry (FC) 4989
Flow cytometric analysis of Human VNN2 expression on human whole blood monocytes (left panel) and granulocytes (right panel). Cells were stained with purified anti-Human VNN2, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes and granulocytes.
Anti-VNN2 Antibody Alternative Names
Anti-FOAP-4 Antibody;Anti-GPI-80 Antibody;Anti-VNN2 Antibody
VNN2 Background Information

Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-80, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.

Full Name
vanin 2
References
  • Suzuki K. et al., 1999, J. Immunol. 162: 4277-84.
  • Martin, F. et al., 2001,Immunogenetics. 53 (4):296-306.
  • Liu T.et al., 2005, J. Proteome Res. 4: 2070-80.
  • Jansen P.AM. et al., 2009, J. Invest. Dermatol. 129: 2167-74.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-VNN2 Antibody Rabbit Polyclonal WB,ELISA 11728-RP02
    Anti-VNN2 Antibody Rabbit Monoclonal ELISA 11728-R018
    Anti-VNN2 Antibody (FITC) Mouse Monoclonal FCM 11728-MM04-F
    Anti-VNN2 Antibody (PE) Mouse Monoclonal FCM 11728-MM04-P
    Anti-VNN2 Antibody Rabbit Polyclonal ELISA 11728-RP01
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