Mouse VNN1 Gene cDNA Clone / ORF Clone

VNN1 cDNA Clone Datasheet

VNN1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011704.3
• cDNA Size: 1539
• Gene Synonym: V-1; Vnn1
• Source: Mouse

VNN1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus vanin 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for twp point mutations:1015 T/C resulting in the amino acid Phe substitution by Leu, 66 T/C not causing the amino acid variation.

VNN1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VNN1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VNN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCATGT CTTGGTGGCT GGCGTGTGCT GCAGCGTTTT CTGCCCTCTG TGTCTTAAAA
61       GCCAGCTCGC TGGATACTTT CCTCGCGGCT GTTTACGAGC ATGCTGTGAT CCTGCCTAAG
121      GACACCCTGT TGCCAGTGTC TCACAGTGAG GCTCTGGCAT TAATGAACCA GAATCTGGAC
181      CTTCTGGAAG GAGCGATCGT ATCTGCAGCG AAGCAGGGTG CGCACATTAT TGTGACTCCA
241      GAAGATGGCA TATACGGTGT GCGTTTCACC AGGGATACGA TCTACCCATA CCTGGAGGAG
301      ATCCCAGACC CTCAAGTAAA CTGGATACCC TGTGATAACC CTAAAAGATT TGGCTCTACC
361      CCGGTGCAGG AGAGACTCAG CTGCTTGGCC AAGAACAACT CCATCTATGT TGTGGCGAAC
421      ATGGGAGACA AGAAGCCGTG TAACACCAGC GACTCTCACT GTCCACCTGA CGGCAGATTC
481      CAGTACAACA CTGATGTGGT GTTTGATTCC CAGGGTAAAC TGGTTGCGAG ATACCATAAG
541      CAAAACATTT TCATGGGAGA AGATCAGTTC AATGTCCCCA TGGAGCCTGA GTTTGTGACT
601      TTCGACACCC CCTTTGGAAA GTTTGGCGTC TTCACCTGTT TCGATATTCT CTTCCATGAT
661      CCCGCTGTCA CCCTGGTGAC AGAATTCCAG GTGGACACCA TACTGTTCCC AACCGCCTGG
721      ATGGACGTCC TTCCTCATTT GGCAGCCATT GAATTCCACT CAGCTTGGGC TATGGGCATG
781      GGGGTCAATT TCCTAGCAGC TAATCTACAT AATCCCTCGA GGAGAATGAC AGGAAGTGGT
841      ATCTATGCAC CCGATTCTCC AAGGGTCTTT CACTACGACA GGAAGACCCA AGAAGGAAAA
901      CTCCTCTTCG CTCAGCTGAA ATCCCACCCA ATTCACTCCC CGGTGAACTG GACTTCCTAT
961      GCTAGCAGTG TAGAATCAAC CCCAACCAAA ACCCAGGAAT TTCAGAGTAT TGTCCTTTTT
1021     GATGAGTTTA CCTTTGTGGA GCTCAAAGGG ATCAAAGGAA ATTACACTGT TTGCCAGAAT
1081     GACCTCTGCT GTCACCTAAG CTACCAGATG TCTGAGAAGC GAGCAGATGA GGTTTATGCC
1141     TTTGGAGCCT TTGATGGGCT GCACACCGTG GAAGGGCAGT ACTACCTACA GATCTGCATC
1201     CTGCTAAAAT GTAAAACTAC CAATTTACGC ACCTGTGGTA GTTCAGTGGA CACGGCTTTT
1261     ACCAGGTTTG AAATGTTCTC GCTCAGCGGC ACTTTTGGAA CCCGGTATGT CTTCCCTGAA
1321     GTGTTGCTGA GTGAGGTCAA GCTCGCACCT GGGGAGTTTC AGGTGTCAAG TGATGGGCGC
1381     CTGGTTAGCC TGAAGCCAAC CTCGGGACCT GTGTTAACCA TCGGGCTCTT TGGGAGGTTG
1441     TATGGGAAGG ACTGGGCATC CAATGCTTCC TCAGACTTCA TAGCACACTC GCTGATAATA
1501     ATGCTGATTG TGACGCCTAT TATACATTAC TTGTGCTGA

 

Quality Control:cDNA sequencing results is available upon request.