VNN1 / Vanin-1 Protein (His Tag)

Pantetheinase / VNN1 / Vanin-1 Protein

Vanin-1 / VNN1 Protein Product Information

• Synonym: VNN1, HDLCQ8, MGC116930, MGC116931, MGC116932, MGC116933, Tiff66
• Protein Construction: A DNA sequence encoding the human VNN1 ( NP_004657.2 ) without the propeptide ( Met 1 - Ser 490 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

Vanin-1 / VNN1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 22
• Molecular Mass: The recombinant human VNN1 consists of 480 amino acids and has a predicted molecular mass of 53.7 kDa. It migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: VNN1 protein

Vanin-1 / VNN1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Vanin-1 / VNN1 Protein Description

Pantetheinase, also known as Pantetheine hydrolase, Vascular non-inflammatory molecule 1, Vanin-1, and VNN1, is a cell membrane protein which belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 contains one CN hydrolase domain. It is widely expressed with higher expression in spleen, kidney and blood. It is overexpressed in lesional psoriatic skin. Vanin-1 is also a member of the Vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sublethal irradiation.

References

1. Aurrand-Lions, M. et al.,1996,  Immunity. 5 (5): 391- 405.
2. Grimmond,S. et al., 2000, Hum Mol Genet 9 (10):1553-60.
3. Meghari,S. et al., 2007, Eur J Immunol. 37 (1): 24-32.
4. Johnson, KA. et al.,2008, Am J Pathol. 172 (2): 440-53.
5. Pouyet, L. et al., 2010, Inflamm Bowel Dis. 16 (1): 96-104.