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Human VIP Gene cDNA Clone (full-length ORF Clone)
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Human VIP Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13451-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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VIP cDNA Clone Datasheet

 

VIP cDNA Clone Product Information

Gene Bank Ref.ID: BC009794

cDNA Size:

510

Gene Synonym:

PHM27; VIP

Source: Human

VIP cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens vasoactive intestinal peptide DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

VIP cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VIP cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VIP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACACCA GAAATAAGGC CCAGCTCCTT GTGCTCCTGA CTCTTCTCAG TGTGCTCTTC
61       TCACAGACTT CGGCATGGCC TCTTTACAGG GCACCTTCTG CTCTCAGGTT GGGTGACAGA
121      ATACCCTTTG AGGGAGCAAA TGAACCTGAT CAAGTTTCAT TAAAAGAAGA CATTGACATG
181      TTGCAAAATG CATTAGCTGA AAATGACACA CCCTATTATG ATGTATCCAG AAATGCCAGG
241      CATGCTGATG GAGTTTTCAC CAGTGACTTC AGTAAACTCT TGGGTCAACT TTCTGCCAAA
301      AAGTACCTTG AGTCTCTTAT GGGAAAACGT GTTAGTAACA TCTCAGAAGA CCCTGTACCA
361      GTCAAACGTC ACTCAGATGC AGTCTTCACT GACAACTATA CCCGCCTTAG AAAACAAATG
421      GCTGTAAAGA AATATTTGAA CTCAATTCTG AATGGAAAGA GGAGCAGTGA GGGAGAATCT
481      CCCGACTTTC CAGAAGAGTT AGAAAAATGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"