Human VIP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13451-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

VIP cDNA Clone Datasheet

VIP cDNA Clone Product Information

Gene Bank Ref.ID:	BC009794
cDNA Size:	510
Gene Synonym:	PHM27; VIP
Source:	Human

VIP cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens vasoactive intestinal peptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

VIP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VIP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VIP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACACCA GAAATAAGGC CCAGCTCCTT GTGCTCCTGA CTCTTCTCAG TGTGCTCTTC
61       TCACAGACTT CGGCATGGCC TCTTTACAGG GCACCTTCTG CTCTCAGGTT GGGTGACAGA
121      ATACCCTTTG AGGGAGCAAA TGAACCTGAT CAAGTTTCAT TAAAAGAAGA CATTGACATG
181      TTGCAAAATG CATTAGCTGA AAATGACACA CCCTATTATG ATGTATCCAG AAATGCCAGG
241      CATGCTGATG GAGTTTTCAC CAGTGACTTC AGTAAACTCT TGGGTCAACT TTCTGCCAAA
301      AAGTACCTTG AGTCTCTTAT GGGAAAACGT GTTAGTAACA TCTCAGAAGA CCCTGTACCA
361      GTCAAACGTC ACTCAGATGC AGTCTTCACT GACAACTATA CCCGCCTTAG AAAACAAATG
421      GCTGTAAAGA AATATTTGAA CTCAATTCTG AATGGAAAGA GGAGCAGTGA GGGAGAATCT
481      CCCGACTTTC CAGAAGAGTT AGAAAAATGA

Quality Control:cDNA sequencing results is available upon request.