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Your Position: Home > Gene Products > Other Species > Danio rerio (zebrafish) VEGFAA Gene cDNA Clone / ORF Clone

Danio rerio (zebrafish) VEGFAA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
ZG63004-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

VEGFAA cDNA Clone Datasheet

VEGFAA cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001110349.2
cDNA Size:	567
Gene Synonym:	vegf; vegfa; wu:fj82c06; vegfaa
Source:	Danio rerio (zebrafish)

VEGFAA cDNA Clone Description

cDNA Description:	ORF Clone of Danio rerio (zebrafish) vascular endothelial growth factor Aa DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

VEGFAA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VEGFAA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VEGFAA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTTGG TTGTTTATTT GATACAGTTA TTTCTCGCGG CTCTCCTCCA TCTGTCTGCT
61       GTAAAGGCTG CCCACATACC CAAAGAAGGG GGAAAGAGCA AAAATGATGT GATTCCCTTC
121      ATGGATGTGT ATAAAAAGAG TGCGTGCAAG ACCCGAGAGC TGCTGGTAGA CATCATCCAG
181      GAGTATCCCG ATGAGATCGA GCACACGTAC ATCCCGTCCT GTGTGGTTCT CATGCGCTGT
241      GCAGGATGCT GTAATGATGA GGCGCTCGAA TGCGTCCCGA CAGAGACACG AAACGTCACT
301      ATGGAGGTGC TGCGGGTCAA GCAACGCGTA TCGCAGCATA ATTTTCAGCT GAGTTTCACA
361      GAACACACCA AGTGTGAATG CAGGCCAAAG GCAGAAGTCA AAGCAAAGAA AGAAAACCAC
421      TGTGAGCCTT GCTCAGAGAG AAGGAAGCGC TTGTATGTGC AGGACCCCCT CACCTGTAAA
481      TGCTCCTGCA AATTCACACA AATGCAATGC AAGTCCAGAC AACTTGAGTT AAACGAAAGA
541      ACTTGCAGAT GTGAAAAGCC AAGATGA

Quality Control:cDNA sequencing results is available upon request.