Human VEGFA transcript variant 4 ORF Expression Clone, His-tagged

VEGFA cDNA Clone Datasheet

VEGFA cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001171626.1
• cDNA Size: 609
• Gene Synonym: VEGF165; VPF; VEGF; MVCD1
• Source: Human

VEGFA cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens vascular endothelial growth factor A transcript variant 4 DNA.
• Vector: pCMV/hygro-His
• His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

VEGFA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VEGFA cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VEGFA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTTTC TGCTGTCTTG GGTGCATTGG AGCCTTGCCT TGCTGCTCTA CCTCCACCAT
61       GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGAG GGCAGAATCA TCACGAAGTG
121      GTGAAGTTCA TGGATGTCTA TCAGCGCAGC TACTGCCATC CAATCGAGAC CCTGGTGGAC
181      ATCTTCCAGG AGTACCCTGA TGAGATCGAG TACATCTTCA AGCCATCCTG TGTGCCCCTG
241      ATGCGATGCG GGGGCTGCTG CAATGACGAG GGCCTGGAGT GTGTGCCCAC TGAGGAGTCC
301      AACATCACCA TGCAGATTAT GCGGATCAAA CCTCACCAAG GCCAGCACAT AGGAGAGATG
361      AGCTTCCTAC AGCACAACAA ATGTGAATGC AGACCAAAGA AAGATAGAGC AAGACAAGAA
421      AATCCCTGTG GGCCTTGCTC AGAGCGGAGA AAGCATTTGT TTGTACAAGA TCCGCAGACG
481      TGTAAATGTT CCTGCAAAAA CACAGACTCG CGTTGCAAGG CGAGGCAGCT TGAGTTAAAC
541      GAACGTACTT GCAGATGTGA CAAGCCGAGG CGGGCT1CACC ATCACCACCA TCATCACCAC
601      CATCAC2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. His Tag

 

 

Quality Control:cDNA sequencing results is available upon request.