Rat VEGF164 / VEGFA Protein (Native)

Vascular endothelial growth factor A Protein Datasheet

VEGF164 / vascular endothelial growth factor A Protein Product Information

• Synonym: Vegfa, VEGF164, Vegf
• Protein Construction: A DNA sequence encoding the rat VEGF 164 isoform (AAA41211.1) (Met 1- Arg 190 ) was expressed.
• Source: Rat
• Expression Host: Baculovirus-Insect cells

VEGF164 / vascular endothelial growth factor A Protein QC Testing

• Purity: > 86 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 27
• Molecular Mass: The secreted recombinant rat VEGF164 consists of 164 amino acids and has a predicted molecular mass of 19.2 kDa. The apparent molecular mass of the rat VEGF164 is approximately 25 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, pH7.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: VEGF164 / vascular endothelial growth factor A protein

VEGF164 / vascular endothelial growth factor A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

VEGF164 / vascular endothelial growth factor A Protein Description

Vascular endothelial growth factor (VEGF) was recently identified as a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. VEGF is a multifunctional cytokine that contributes to angiogenesis by both direct and indirect mechanisms. On the one hand, VEGF stimulates the ECs lining nearby microvessels to proliferate, migrate, and to alter their pattern of gene expression. On the other hand, VEGF renders these same microvascular ECs hyperpermeable so that they spill plasma proteins into the extravascular space, leading to the clotting of extravasated fibrinogen with deposition of a fibrin gel. Molecular cloning revealed multiple forms of VEGF, apparently arising from alternative splicing of its RNA transcript. Vascular endothelial growth factor A（VEGFA）is one member of VEGF family. VEGFA is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Protein levels of vascular endothelial growth factor-A (VEGF-A) is elevated in Indian Amyotrophic Lateral Sclerosis (ALS) patients.

References

1. Plate KH. et al., 1992, Nature. 359: 845-8.
2. Dvorak HF. et al., 1995, Am J Pathol. 146 (5): 1029-39.
3. Leung DW. et al., 1989, Science . 246 (4935) : 1306-9.
4. Alon T. et al., 1995, Nature Medicine 1: 1024-8.
5. Jalb CS. et al., 2011, Rom J Morphol Embryol. 52 (3): 775-81.