Mouse VEGF-D / VEGFD / FIGF Protein

C-fos induced growth factor Protein Datasheet

VEGF-D / VEGFD / FIGF Protein Product Information

• Synonym: RP23-202H24.2, AI325264, VEGF-D, Vegfd
• Protein Construction: A DNA sequence encoding the mature form of mouse FIGF (P97946) (Phe 98-Ser 206) was fused with the Fc region of human IgG1 at the N-terminus.
• Source: Mouse
• Expression Host: Human Cells

VEGF-D / VEGFD / FIGF Protein QC Testing

• Purity: (65.7+30.1) % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized human VEGFR3-His (Cat:10806-H08H) at 10 μg/mL (100 μl/well) can bind mouse Fc-VEGFD, The EC₅₀ of mouse Fc-VEGFD is 49 ng/mL.
  3. Immobilized mouse VEGFR3-His (Cat:50584-M08H) at 10 μg/mL (100 μl/well) can bind mouse Fc-VEGFD, The EC₅₀ of mouse Fc-VEGFD is 44 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant mouse FIGF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 369 amino acids and has a calculated molecular mass of 40.6 kDa.The apparent molecular mass of the protein is approximately 45 and 36 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: VEGF-D / VEGFD / FIGF protein

VEGF-D / VEGFD / FIGF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

VEGF-D / VEGFD / FIGF Protein Description

Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), is a vascular endothelial growth factor. This secreted protein is a member of the platelet derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and performs activities in angiogenesis, lymphangiogenesis, and endothelial cell growth. Human VEGF-D is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. It is structurally and functionally similar to VEGF-C, and is secreted as a non-covelent homodimer in a antiparallel fashion. VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. Like VEGFC, it may particularly be involed in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults.

References

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6. Otrock ZH. et al., 2007, Blood Cells Mol Dis. 38: 258-68.