VCL / Vinculin Protein Price Inquiry ( Available Sizes )
VCL / Vinculin Protein Product Information
||RP11-178G16.3, CMD1W, CMH15, MVCL
||A DNA sequence encoding the human VCL (P18206-2) (Met 1- Gln 1066) was expressed, fused with a polyhistidine tag at the C-terminus.
VCL / Vinculin Protein QC Testing
||> 90 % as determined by SDS-PAGE.
VCL / Vinculin protein
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||The recombinant human VCL consists of 1077 amino acids and has a calculated molecular mass of 118 KDa. The apparent molecular mass of rhVCL is approximately 115 KDa in SDS-PAGE under reducing conditions.
||Lyophilized from sterile PBS , pH 7.4
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
VCL / Vinculin Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
VCL / Vinculin Protein Related Products & Topics
VCL / Vinculin Protein Description
Vinculin (VCL), is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, paxillin and a-catenin, which reportedly bind to either the head or tail domains of vinculin. Moreover, it is believed that vinculin can serve as a dynamic link between the cytoskeleton and cell surface adhesion molecules, such as integrins and E-cadherins. Vinculin has also been implicated in the control of adhesion and motility of fibroblasts, PC12 cells and chick neuronal cells.
- Massoumi R. et al., 2001, J Cell Sci. 114 (10): 1925-34.
- Turner CE. et al., 1994, J Cell Sci. 107 (6): 1583-91.
- Strasser P. et al., 1993, FEBS Lett. 317: 189-194.