Human VASN Gene cDNA Clone / ORF Clone

VASN cDNA Clone Datasheet

VASN cDNA Clone Product Information

• Gene Bank Ref.ID: BC068575
• cDNA Size: 2022
• Gene Synonym: UNQ314/PRO357/PRO1282, SLITL2,VASN
• Source: Human

VASN cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens vasorin DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1446 C/T not causing the amino acid variation.

VASN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

VASN cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

VASN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGCTCCA GGGTCCCTCT GCTGCTGCCG CTGCTCCTGC TACTGGCCCT GGGGCCTGGG
61       GTGCAGGGCT GCCCATCCGG CTGCCAGTGC AGCCAGCCAC AGACAGTCTT CTGCACTGCC
121      CGCCAGGGGA CCACGGTGCC CCGAGACGTG CCACCCGACA CGGTGGGGCT GTACGTCTTT
181      GAGAACGGCA TCACCATGCT CGACGCAGGC AGCTTTGCCG GCCTGCCGGG CCTGCAGCTC
241      CTGGACCTGT CACAGAACCA GATCGCCAGC CTGCCCAGCG GGGTCTTCCA GCCACTCGCC
301      AACCTCAGCA ACCTGGACCT GACGGCCAAC AGGCTGCATG AAATCACCAA TGAGACCTTC
361      CGTGGCCTGC GGCGCCTCGA GCGCCTCTAC CTGGGCAAGA ACCGCATCCG CCACATCCAG
421      CCTGGTGCCT TCGACACGCT CGACCGCCTC CTGGAGCTCA AGCTGCAGGA CAACGAGCTG
481      CGGGCACTGC CCCCGCTGCG CCTGCCCCGC CTGCTGCTGC TGGACCTCAG CCACAACAGC
541      CTCCTGGCCC TGGAGCCCGG CATCCTGGAC ACTGCCAACG TGGAGGCGCT GCGGCTGGCT
601      GGTCTGGGGC TGCAGCAGCT GGACGAGGGG CTCTTCAGCC GCTTGCGCAA CCTCCACGAC
661      CTGGATGTGT CCGACAACCA GCTGGAGCGA GTGCCACCTG TGATCCGAGG CCTCCGGGGC
721      CTGACGCGCC TGCGGCTGGC CGGCAACACC CGCATTGCCC AGCTGCGGCC CGAGGACCTG
781      GCCGGCCTGG CTGCCCTGCA GGAGCTGGAT GTGAGCAACC TAAGCCTGCA GGCCCTGCCT
841      GGCGACCTCT CGGGCCTCTT CCCCCGCCTG CGGCTGCTGG CAGCTGCCCG CAACCCCTTC
901      AACTGCGTGT GCCCCCTGAG CTGGTTTGGC CCCTGGGTGC GCGAGAGCCA CGTCACACTG
961      GCCAGCCCTG AGGAGACGCG CTGCCACTTC CCGCCCAAGA ACGCTGGCCG GCTGCTCCTG
1021     GAGCTTGACT ACGCCGACTT TGGCTGCCCA GCCACCACCA CCACAGCCAC AGTGCCCACC
1081     ACGAGGCCCG TGGTGCGGGA GCCCACAGCC TTGTCTTCTA GCTTGGCTCC TACCTGGCTT
1141     AGCCCCACAG CGCCGGCCAC TGAGGCCCCC AGCCCGCCCT CCACTGCCCC ACCGACTGTA
1201     GGGCCTGTCC CCCAGCCCCA GGACTGCCCA CCGTCCACCT GCCTCAATGG GGGCACATGC
1261     CACCTGGGGA CACGGCACCA CCTGGCGTGC TTGTGCCCCG AAGGCTTCAC GGGCCTGTAC
1321     TGTGAGAGCC AGATGGGGCA GGGGACACGG CCCAGCCCTA CACCAGTCAC GCCGAGGCCA
1381     CCACGGTCCC TGACCCTGGG CATCGAGCCG GTGAGCCCCA CCTCCCTGCG CGTGGGGCTG
1441     CAGCGTTACC TCCAGGGGAG CTCCGTGCAG CTCAGGAGCC TCCGTCTCAC CTATCGCAAC
1501     CTATCGGGCC CTGATAAGCG GCTGGTGACG CTGCGACTGC CTGCCTCGCT CGCTGAGTAC
1561     ACGGTCACCC AGCTGCGGCC CAACGCCACT TACTCCGTCT GTGTCATGCC TTTGGGGCCC
1621     GGGCGGGTGC CGGAGGGCGA GGAGGCCTGC GGGGAGGCCC ATACACCCCC AGCCGTCCAC
1681     TCCAACCACG CCCCAGTCAC CCAGGCCCGC GAGGGCAACC TGCCGCTCCT CATTGCGCCC
1741     GCCCTGGCCG CGGTGCTCCT GGCCGCGCTG GCTGCGGTGG GGGCAGCCTA CTGTGTGCGG
1801     CGGGGGCGGG CCATGGCAGC AGCGGCTCAG GACAAAGGGC AGGTGGGGCC AGGGGCTGGG
1861     CCCCTGGAAC TGGAGGGAGT GAAGGTCCCC TTGGAGCCAG GCCCGAAGGC AACAGAGGGC
1921     GGTGGAGAGG CCCTGCCCAG CGGGTCTGAG TGTGAGGTGC CACTCATGGG CTTCCCAGGG
1981     CCTGGCCTCC AGTCACCCCT CCACGCAAAG CCCTACATCT AA

 

Quality Control:cDNA sequencing results is available upon request.