>Ultra linked Protein A resin
Ultra linked Protein A resin
Sino Biological Ultra-linked rProtein A resin is a high capacity, high throughput and low leaching medium for purification of monoclonal or polyclonal antibodies. The resin is stable, durable, cost-effective, and suitable for both pilot and large scale purification.
High Binding Capacity
As a result of the special coupling methods, Sino Biological Ultra-linked rProtein A resin provides a high binding capacity, with a dynamic binding capacity over 30 mg for human IgG.
|Antibody loaded (mg)||recovery %|
Antibody recovery in eluant when specific anmount was applied on 1 ml column
Binding buffer: 50mM Tris, 100mM NaCl, pH8.0
Elution buffer: 100mM Gly, 50mM NaCl, pH3.0
Column bed volume: 1 ml; Flow rate: 0.2 ml/min
Sino Biological Ultra-linked rProtein A resin was produced by immobilizing recombinant protein A to 4% cross-linked agarose matrix. The matrix is highly cross-linked, physically and chemically stable, and particularly suitable for process scale applications where starting material volumes are large and flow rates are high. The maximum flow rate is 250 cm/h.
Pressure / flow rate curve (1.3*20cm,CV=26.5ml,water)
Low Protein A Leaching
The ligand protein A has been coupled to the matrix through a stable covalent bond . And the leached protein A ligand is far below 100 ppm.
The column was loaded with antibody at 30mg/ml for 5min. The antibody recovery remains over 96%, after 30 cycles of CIP.
One CIP cycle includes the following washing steps: Washing: 0.05M NaOH, 1 M NaCl, 0.2ml/min, 6 column volumes (CV), Balancing: 5 CV, Eluting: 5 CV
|rProtein G resin|
|rProtein L resin|
|Recombinant Protein A|
|Recombinant Protein G|
|Recombinant Protein L|