USP46 Protein (N-SUMO)

Ubiquitin specific peptidase 46 Protein Datasheet

USP46 Protein Product Information

• Synonym: USP46
• Protein Construction: A DNA sequence encoding the human USP46 (P80075-1) (Met1-Glu366) was fused with the SUMO tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

USP46 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The secreted recombinant human USP46 consists of 477 amino acids and predicts a molecular mass of 55.2 KDa. The apparent molecular mass of the protein is approximately 64 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: USP46 protein

USP46 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

USP46 Protein Description

USP46 is a member of the peptidase C19 family, USP12/USP46 subfamily. USP46 is a deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. Deubiquitinating enzymes (DUBs) are a large group of proteases which are also commonly referred to as deubiquitinating peptidases, deubiquitinating isopeptidases, deubiquitinases, ubiquitin proteases, ubiquitin hydrolyases, ubiquitin isopeptidases, or Dubs. They regulate ubiquitin-dependent metabolic pathways by cleaving ubiquitin-protein bonds. They also may act as negative and positive regulators of the ubiquitin system. USP46 is broadly expressed. It may act by mediating the deubiquitination of GAD1/GAD67. USP46 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity and it is not involved in deubiquitination of monoubiquitinated FANCD2.

References

1. Joo HY. et al., 2011, J Biol Chem. 286 (9): 7190-201.
2. Kushima I. et al., 2010, J Hum Genet. 55 (3): 133-6.
3. Cohn MA. et al., 2009, J Biol Chem. 284 (8): 5343-51.