Human UNC5B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13606-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

UNC5B cDNA Clone Datasheet

UNC5B cDNA Clone Product Information

Gene Bank Ref.ID:	NM_170744.4
cDNA Size:	2838
Gene Synonym:	UNC5H2; p53RDL1;UNC5B
Source:	Human

UNC5B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens unc-5 homolog B (C. elegans) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

UNC5B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UNC5B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UNC5B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGCCC GGAGCGGAGC TCGGGGCGCG CTGCTGCTGG CACTGCTGCT CTGCTGGGAC
61       CCGAGGCTGA GCCAAGCAGG CACTGATTCT GGCAGCGAGG TGCTCCCTGA CTCCTTCCCG
121      TCAGCGCCAG CAGAGCCGCT GCCCTACTTC CTGCAGGAGC CACAGGACGC CTACATTGTG
181      AAGAACAAGC CTGTGGAGCT CCGCTGCCGC GCCTTCCCCG CCACACAGAT CTACTTCAAG
241      TGCAACGGCG AGTGGGTCAG CCAGAACGAC CACGTCACAC AGGAAGGCCT GGATGAGGCC
301      ACCGGCCTGC GGGTGCGCGA GGTGCAGATC GAGGTGTCGC GGCAGCAGGT GGAGGAGCTC
361      TTTGGGCTGG AGGATTACTG GTGCCAGTGC GTGGCCTGGA GCTCCGCGGG CACCACCAAG
421      AGTCGCCGAG CCTACGTCCG CATCGCCTAC CTGCGCAAGA ACTTCGATCA GGAGCCTCTG
481      GGCAAGGAGG TGCCCCTGGA CCATGAGGTT CTCCTGCAGT GCCGCCCGCC GGAGGGGGTG
541      CCTGTGGCCG AGGTGGAATG GCTCAAGAAT GAGGATGTCA TCGACCCCAC CCAGGACACC
601      AACTTCCTGC TCACCATCGA CCACAACCTC ATCATCCGCC AGGCCCGCCT GTCGGACACT
661      GCCAACTATA CCTGCGTGGC CAAGAACATC GTGGCCAAAC GCCGGAGCAC CACTGCCACC
721      GTCATCGTCT ACGTGAATGG CGGCTGGTCC AGCTGGGCAG AGTGGTCACC CTGCTCCAAC
781      CGCTGTGGCC GAGGCTGGCA GAAGCGCACC CGGACCTGCA CCAACCCCGC TCCACTCAAC
841      GGAGGGGCCT TCTGCGAGGG CCAGGCATTC CAGAAGACCG CCTGCACCAC CATCTGCCCA
901      GTCGATGGGG CGTGGACGGA GTGGAGCAAG TGGTCAGCCT GCAGCACTGA GTGTGCCCAC
961      TGGCGTAGCC GCGAGTGCAT GGCGCCCCCA CCCCAGAACG GAGGCCGTGA CTGCAGCGGG
1021     ACGCTGCTCG ACTCTAAGAA CTGCACAGAT GGGCTGTGCA TGCAAAATAA GAAAACTCTA
1081     AGCGACCCCA ACAGCCACCT GCTGGAGGCC TCAGGGGATG CGGCGCTGTA TGCGGGGCTC
1141     GTGGTGGCCA TCTTCGTGGT CGTGGCAATC CTCATGGCGG TGGGGGTGGT GGTGTACCGC
1201     CGCAACTGCC GTGACTTCGA CACAGACATC ACTGACTCAT CTGCTGCCCT GACTGGTGGT
1261     TTCCACCCCG TCAACTTTAA GACGGCAAGG CCCAGCAACC CGCAGCTCCT ACACCCCTCT
1321     GTGCCTCCTG ACCTGACAGC CAGCGCCGGC ATCTACCGCG GACCCGTGTA TGCCCTGCAG
1381     GACTCCACCG ACAAAATCCC CATGACCAAC TCTCCTCTGC TGGACCCCTT ACCCAGCCTT
1441     AAGGTCAAGG TCTACAGCTC CAGCACCACG GGCTCTGGGC CAGGCCTGGC AGATGGGGCT
1501     GACCTGCTGG GGGTCTTGCC GCCTGGCACA TACCCTAGCG ATTTCGCCCG GGACACCCAC
1561     TTCCTGCACC TGCGCAGCGC CAGCCTCGGT TCCCAGCAGC TCTTGGGCCT GCCCCGAGAC
1621     CCAGGGAGCA GCGTCAGCGG CACCTTTGGC TGCCTGGGTG GGAGGCTCAG CATCCCCGGC
1681     ACAGGGGTCA GCTTGCTGGT GCCCAATGGA GCCATTCCCC AGGGCAAGTT CTACGAGATG
1741     TATCTACTCA TCAACAAGGC AGAAAGTACC CTCCCGCTTT CAGAAGGGAC CCAGACAGTA
1801     TTGAGCCCCT CGGTGACCTG TGGACCCACA GGCCTCCTGC TGTGCCGCCC CGTCATCCTC
1861     ACCATGCCCC ACTGTGCCGA AGTCAGTGCC CGTGACTGGA TCTTTCAGCT CAAGACCCAG
1921     GCCCACCAGG GCCACTGGGA GGAGGTGGTG ACCCTGGATG AGGAGACCCT GAACACACCC
1981     TGCTACTGCC AGCTGGAGCC CAGGGCCTGT CACATCCTGC TGGACCAGCT GGGCACCTAC
2041     GTGTTCACGG GCGAGTCCTA TTCCCGCTCA GCAGTCAAGC GGCTCCAGCT GGCCGTCTTC
2101     GCCCCCGCCC TCTGCACCTC CCTGGAGTAC AGCCTCCGGG TCTACTGCCT GGAGGACACG
2161     CCTGTAGCAC TGAAGGAGGT GCTGGAGCTG GAGCGGACTC TGGGCGGATA CTTGGTGGAG
2221     GAGCCGAAAC CGCTAATGTT CAAGGACAGT TACCACAACC TGCGCCTCTC CCTCCATGAC
2281     CTCCCCCATG CCCATTGGAG GAGCAAGCTG CTGGCCAAAT ACCAGGAGAT CCCCTTCTAT
2341     CACATTTGGA GTGGCAGCCA GAAGGCCCTC CACTGCACTT TCACCCTGGA GAGGCACAGC
2401     TTGGCCTCCA CAGAGCTCAC CTGCAAGATC TGCGTGCGGC AAGTGGAAGG GGAGGGCCAG
2461     ATATTCCAGC TGCATACCAC TCTGGCAGAG ACACCTGCTG GCTCCCTGGA CACTCTCTGC
2521     TCTGCCCCTG GCAGCACTGT CACCACCCAG CTGGGACCTT ATGCCTTCAA GATCCCACTG
2581     TCCATCCGCC AGAAGATATG CAACAGCCTA GATGCCCCCA ACTCACGGGG CAATGACTGG
2641     CGGATGTTAG CACAGAAGCT CTCTATGGAC CGGTACCTGA ATTACTTTGC CACCAAAGCG
2701     AGCCCCACGG GTGTGATCCT GGACCTCTGG GAAGCTCTGC AGCAGGACGA TGGGGACCTC
2761     AACAGCCTGG CGAGTGCCTT GGAGGAGATG GGCAAGAGTG AGATGCTGGT GGCTGTGGCC
2821     ACCGACGGGG ACTGCTGA

Quality Control:cDNA sequencing results is available upon request.