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Human UGT1A4 Gene cDNA Clone (full-length ORF Clone)
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Human UGT1A4 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13315-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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UGT1A4 cDNA Clone Datasheet

 

UGT1A4 cDNA Clone Product Information

Gene Bank Ref.ID: NM_007120.2

cDNA Size:

1605

Gene Synonym:

UDPGT; UGT1D; UGT-1D; UGT1.4; HUG-BR2; UGT1-04; UDPGT 1-4; UGT1A4

Source: Human

UGT1A4 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens UDP glucuronosyltransferase 1 family, polypeptide A4 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 142 T/G, 986 A/G resulting in the amino acid Leu substitution by Val, Lys substitution by Arg and 471 T/C not causing the amino acid variation.

UGT1A4 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UGT1A4 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UGT1A4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAGAG GACTCCAGGT TCCCCTGCCG CGGCTGGCCA CAGGACTGCT GCTCCTCCTC
61       AGTGTCCAGC CCTGGGCTGA GAGTGGAAAG GTGTTGGTGG TGCCCACTGA TGGCAGCCCC
121      TGGCTCAGCA TGCGGGAGGC CGTGCGGGAG CTCCATGCCA GAGGCCACCA GGCGGTGGTC
181      CTCACCCCAG AGGTGAATAT GCACATCAAA GAAGAGAAAT TTTTCACCCT GACAGCCTAT
241      GCTGTTCCAT GGACCCAGAA GGAATTTGAT CGCGTTACGC TGGGCTACAC TCAAGGGTTC
301      TTTGAAACAG AACATCTTCT GAAGAGATAT TCTAGAAGTA TGGCAATTAT GAACAATGTA
361      TCTTTGGCCC TTCATAGGTG TTGTGTGGAG CTACTGCATA ATGAGGCCCT GATCAGGCAC
421      CTGAATGCTA CTTCCTTTGA TGTGGTTTTA ACAGACCCCG TTAACCTCTG CGGGGCGGTG
481      CTGGCTAAGT ACCTGTCGAT TCCTGCTGTG TTTTTTTGGA GGTACATTCC ATGTGACTTA
541      GACTTTAAGG GCACACAGTG TCCAAATCCT TCCTCCTATA TTCCTAAGTT ACTAACGACC
601      AATTCAGACC ACATGACATT CCTGCAAAGG GTCAAGAACA TGCTCTACCC TCTGGCCCTG
661      TCCTACATTT GCCATACTTT TTCTGCCCCT TATGCAAGTC TTGCCTCTGA GCTTTTTCAG
721      AGAGAGGTGT CAGTGGTGGA TCTTGTCAGC TATGCATCCG TGTGGCTGTT CCGAGGGGAC
781      TTTGTGATGG ACTACCCCAG GCCGATCATG CCCAACATGG TCTTCATTGG GGGCATCAAC
841      TGTGCCAACG GGAAGCCACT ATCTCAGGAA TTTGAAGCCT ACATTAATGC TTCTGGAGAA
901      CATGGAATTG TGGTTTTCTC TTTGGGATCA ATGGTCTCAG AAATTCCAGA GAAGAAAGCT
961      ATGGCAATTG CTGATGCTTT GGGCAGAATC CCTCAGACAG TCCTGTGGCG GTACACTGGA
1021     ACCCGACCAT CGAATCTTGC GAACAACACG ATACTTGTTA AGTGGCTACC CCAAAACGAT
1081     CTGCTTGGTC ACCCGATGAC CCGTGCCTTT ATCACCCATG CTGGTTCCCA TGGTGTTTAT
1141     GAAAGCATAT GCAATGGCGT TCCCATGGTG ATGATGCCCT TGTTTGGTGA TCAGATGGAC
1201     AATGCAAAGC GCATGGAGAC TAAGGGAGCT GGAGTGACCC TGAATGTTCT GGAAATGACT
1261     TCTGAAGATT TAGAAAATGC TCTAAAAGCA GTCATCAATG ACAAAAGTTA CAAGGAGAAC
1321     ATCATGCGCC TCTCCAGCCT TCACAAGGAC CGCCCGGTGG AGCCGCTGGA CCTGGCCGTG
1381     TTCTGGGTGG AGTTTGTGAT GAGGCACAAG GGCGCGCCAC ACCTGCGCCC CGCAGCCCAC
1441     GACCTCACCT GGTACCAGTA CCATTCCTTG GACGTGATTG GTTTCCTCTT GGCCGTCGTG
1501     CTGACAGTGG CCTTCATCAC CTTTAAATGT TGTGCTTATG GCTACCGGAA ATGCTTGGGG
1561     AAAAAAGGGC GAGTTAAGAA AGCCCACAAA TCCAAGACCC ATTGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"