Human UBE4B transcript variant 2 Gene cDNA Clone / ORF Clone

UBE4B cDNA Clone Datasheet

UBE4B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006048.4
• cDNA Size: 3522
• Gene Synonym: E4; UFD2; HDNB1; UBOX3; UFD2A;UBE4B
• Source: Human

UBE4B cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ubiquitination factor E4B, transcript variant 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

UBE4B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

UBE4B cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

UBE4B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGGAGC TGAGCGCTGA TGAGATTCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA
61       CAGACCTCTC AGCCAACCAC CCCACTCACC TCTCCCCAGA GGGAGAACCC TCCGGGGCCT
121      CCCATAGCGG CATCAGCCCC AGGACCCTCT CAGAGTCTTG GTCTCAATGT CCACAACATG
181      ACCCCAGCTA CCTCCCCAAT AGGTGCATCA GGAGTAGCCC ATCGAAGCCA GAGCAGTGAA
241      GGAGTCAGTT CTCTCAGCAG CTCGCCCTCT AATAGCCTTG AAACGCAATC TCAGTCTCTC
301      TCACGTTCCC AGAGCATGGA TATCGATGGT GTCTCATGTG AGAAAAGCAT GTCCCAGGTG
361      GATGTGGATT CAGGAATTGA AAACATGGAG GTTGATGAAA ATGATCGAAG AGAAAAGCGG
421      AGCCTCAGTG ATAAGGAGCC TTCCTCGGGC CCTGAAGTGT CTGAAGAGCA GGCCTTACAG
481      CTGGTCTGTA AGATCTTCCG TGTCTCTTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT
541      TCTTCTCTTT CTGCACAGTT TAAGCAGAAC CCAAAAGAAG TATTCTCCGA TTTTAAGGAC
601      TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCCA CTCAGACCAG AGATGAAAAC
661      CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCTG CAGCAGCACG GTCACCAGAC
721      AGAAATCTCT TGCTAAACAC TGGCTCCAAT CCAGGAACAA GCCCCATGTT CTGCAGCGTG
781      GCTTCCTTTG GTGCCAGCTC TTTGTCTAGT TTGGGAGCCT CTGGTGGAGC AAGTAATTGG
841      GATTCCTACA GTGACCATTT CACCATTGAA ACCTGCAAAG AGACAGATAT GCTGAACTAC
901      CTCATCGAGT GTTTCGACCG AGTTGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC
961      CAGCCAGCAG TCAGCCAGCT TCTGAGCAAC ATCCGCTCAC AGTGCATATC CCATACTGCT
1021     TTAGTACTAC AAGGCTCCCT AACACAGCCC AGGTCCTTGC AGCAGCCGTC CTTCCTAGTG
1081     CCGTATATGC TGTGTAGGAA TCTCCCATAT GGCTTCATTC AGGAACTGGT GAGAACCACT
1141     CACCAGGATG AAGAAGTGTT CAAGCAGATA TTTATCCCCA TTTTACAAGG CCTGGCTCTT
1201     GCTGCCAAAG AGTGCTCCCT CGACAGTGAC TACTTTAAAT ACCCCCTCAT GGCACTAGGT
1261     GAGCTCTGTG AAACCAAGTT TGGGAAGACA CACCCTGTGT GCAATTTGGT TGCTTCTTTG
1321     CGGTTGTGGT TGCCGAAATC CTTAAGTCCT GGCTGTGGGC GGGAGCTGCA GAGACTCTCT
1381     TACTTAGGGG CTTTCTTTAG CTTCTCAGTC TTTGCAGAAG ATGATGTTAA AGTGGTTGAA
1441     AAATACTTCT CAGGGCCTGC CATTACCCTG GAAAACACTC GTGTGGTTAG CCAATCATTG
1501     CAGCATTACT TAGAGCTCGG AAGGCAAGAG CTTTTTAAGA TTCTGCATAG TATTTTGTTA
1561     AATGGCGAAA CCCGTGAGGC TGCTCTCAGT TACATGGCGG CTGTCGTCAA TGCCAATATG
1621     AAGAAAGCAC AGATGCAGAC AGATGATAGA TTGGTGTCTA CAGATGGATT TATGCTGAAT
1681     TTCCTTTGGG TACTGCAGCA GCTAAGTACA AAAATCAAGT TAGAAACAGT TGATCCCACG
1741     TATATTTTTC ACCCAAGATG TCGGATTACT CTTCCCAATG ATGAGACGCG TGTGAATGCA
1801     ACGATGGAAG ATGTGAATGA CTGGCTGACT GAACTCTATG GCGATCAGCC TCCATTTTCT
1861     GAGCCGAAAT TCCCTACGGA GTGCTTCTTT CTCACCCTGC ATGCTCACCA CCTCTCTATT
1921     CTGCCTAGTT GCCGTCGCTA TATCCGCAGA CTCCGGGCTA TCCGGGAGCT CAATAGAACT
1981     GTAGAAGATT TGAAAAATAA TGAAAGCCAA TGGAAAGATT CCCCACTGGC AACTAGACAC
2041     CGCGAAATGC TGAAGCGCTG TAAAACTCAG CTTAAGAAAC TGGTACGGTG CAAGGCCTGT
2101     GCTGATGCTG GCCTACTTGA CGAGAGCTTC CTGAGAAGAT GTCTGAATTT TTATGGCCTT
2161     CTCATTCAGC TGCTGCTCCG CATCCTGGAC CCCGCATATC CCGATATAAC ACTGCCTTTA
2221     AATTCAGATG TCCCCAAGGT ATTTGCAGCG TTGCCTGAGT TTTATGTAGA AGATGTTGCA
2281     GAATTTTTAT TTTTTATTGT ACAATACTCT CCCCAGGCGC TTTATGAGCC CTGTACTCAG
2341     GATATTGTGA TGTTCCTTGT TGTGATGTTG TGCAACCAGA ACTACATCCG AAACCCATAT
2401     TTGGTGGCCA AACTGGTAGA AGTCATGTTT ATGACCAACC CTGCTGTTCA GCCACGAACC
2461     CAGAAGTTTT TTGAAATGAT TGAGAACCAT CCTCTCTCCA CCAAGTTGTT GGTACCTTCC
2521     CTGATGAAGT TTTATACAGA TGTTGAGCAT ACCGGAGCCA CCAGTGAGTT TTATGACAAG
2581     TTCACAATTC GCTATCATAT TAGCACCATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC
2641     CATGGCACCT TTATGGAGGA GTTCAACTCC GGGAAGCAGT TTGTTCGCTA TATAAACATG
2701     TTGATAAACG ACACGACGTT TTTGCTCGAT GAAAGTCTGG AGTCTCTGAA GCGAATCCAT
2761     GAAGTGCAGG AAGAGATGAA GAACAAAGAA CAGTGGGACC AGTTGCCCCG GGATCAGCAG
2821     CAGGCTCGTC AGTCTCAGCT TGCTCAGGAT GAGCGTGTGT CCCGCTCTTA CCTCGCCCTG
2881     GCCACCGAAA CCGTGGACAT GTTCCACATC CTCACGAAGC AGGTCCAGAA GCCCTTCCTC
2941     AGACCGGAGC TTGGACCCCG ATTGGCTGCA ATGCTGAACT TTAATCTTCA GCAACTTTGT
3001     GGCCCCAAGT GCCGTGACCT GAAAGTTGAA AACCCTGAGA AATACGGCTT TGAACCAAAG
3061     AAGCTGTTGG ACCAACTGAC GGATATTTAC TTACAGCTGG ACTGTGCTCG GTTCGCGAAA
3121     GCCATTGCTG ACGACCAGAG ATCCTACAGT AAGGAATTGT TTGAAGAAGT TATTTCAAAG
3181     ATGCGGAAGG CAGGGATCAA ATCCACAATA GCAATAGAAA AATTTAAGCT GCTCGCCGAG
3241     AAAGTGGAGG AGATAGTGGC CAAGAACGCA CGCGCAGAAA TCGACTACAG CGACGCTCCT
3301     GATGAGTTCA GAGACCCTCT GATGGACACC CTCATGACAG ACCCCGTGCG GCTGCCCTCT
3361     GGCACCATCA TGGACCGCTC CATCATCCTG CGGCACCTGC TCAACTCCCC CACGGACCCC
3421     TTCAACCGGC AGACGCTGAC AGAGAGCATG CTGGAACCAG TGCCAGAACT GAAAGAGCAG
3481     ATTCAGGCGT GGATGAGAGA GAAACAGAAC AGCGATCACT AA

 

Quality Control:cDNA sequencing results is available upon request.