UBE2M

UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

UBE2M Related Areas

Enzyme>>Protease & Regulator>>Other Proteases>> UBE2M

Neuroscience>>Neurodegenerative Disease>>Ubiquitin-proteasome pathway>> UBE2M

UBE2M Alternative Names

UBC-RS2, UBC12, hUbc12 [Homo sapiens]

2510040H03Rik, UBC12, Ubc-rs2[Mus musculus]

Summaries for UBE2M

Entrez Gene summary for UBE2M:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

Wikipedia summary for UBE2M:

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Human UBE2M Protein General Information

 

• Protein names: Ubiquitin-conjugating enzyme E2 M
  Short name=UBE2M
• Sequence length: 183 AA.
• Domain: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
• Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
• Post-translational modification: The acetylation of Met-1 is cotranslational, and not regulatory. The N-acetylmethionine increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
• Subunit structure: Interacts with UBA3, DCUN1D1 and RBX1.
• Pathway: Protein modification; protein neddylation.
• Catalytic activity: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

General information above from UniProt

Function for UBE2M Protein

UniProtKB:

UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. UBE2M is involved in cell proliferation.

Genatlas:

• UBE2M is putative NEDD8 conjugation enzyme E2(Wada 2000)
• UBE2M ubiquitinates cellular proteins to mark them for degradation (second step in ubiquitin conjugation)
• UBE2M participates in the modification of CUL4A by NEDD8

Homology for human UBE2M

• homolog to Drosophila CG7375
• homolog to yeast S.cerevisiae Ubc12