UBE2I

UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

UBE2I Related Areas

Enzyme>>Protease & Regulator>>Other Proteases>> UBE2I

Neuroscience>>Neurodegenerative Disease>>Ubiquitin-proteasome pathway>> UBE2I

UBE2I Alternative Names

C358B7.1, P18, UBC9 [Homo sapiens]

5830467E05Rik, F830028O17Rik, UBC9, Ubce2i, Ubce9 [Mus musculus]

Summaries for UBE2I

Entrez Gene summary for UBE2I:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for UBE2I gene. [provided by RefSeq, Jul 2008]

Wikipedia summary for UBE2I:

SUMO-conjugating enzyme UBC9 is a protein that in humans is encoded by the UBE2I gene.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Human UBE2I Protein General Information

 

• Protein names: Ubiquitin-conjugating enzyme E2 I
  Short name=UBE2I
• Sequence length: 158 AA.
• Pathway: Protein modification; protein sumoylation.
• Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
• Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.
• Subunit structure: Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation By similarity. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation Probable. Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML-RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains.
• Subcellular location: Nucleus. Cytoplasm. Note: Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
• Tissue specificity: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
• Sequence caution: The sequence AAH51289.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
  The sequence BAD92225.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

General information above from UniProt

Function for UBE2I Protein

UniProtKB:

UBE2I accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. UBE2I can catalyze the formation of poly-SUMO chains. UBE2I is necessary for sumoylation of FOXL2 and KAT5. UBE2I is essential for nuclear architecture and chromosome segregation.