UBE2E1 / UbcH6 Protein

  Ubiquitin-conjugating enzyme E2E 1 Protein Datasheet

UBE2E1 / UbcH6 Protein Product Information

• Synonym: UBCH6
• Protein Construction: A DNA sequence encoding the human UBE2E1 (P51965) (Ser 2- Thr 193) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
• Source: Human
• Expression Host: E.coli

UBE2E1 / UbcH6 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly
• Molecular Mass: The recombinant human UBE2E1 consists of 194 amino acids and has a calculated molecular mass of 21.3 KDa. It migrates as an approximately 24KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, 20%glycerol, , pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: UBE2E1 / UbcH6 protein

UBE2E1 / UbcH6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

UBE2E1 / UbcH6 Protein Description

Ubiquitin-conjugating enzyme E2 E1 (UBE2E1) is a protein localized to the nucleus. The modification of proteins with ubiquitin is an importantly step for targeting abnormal or short-lived proteins for degeneration. Ubiquitination involves three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2E1 is a member of the ubiquitin-donjugating enzyme family. It has been demonstrated to be released to Sj grens's syndrome and systemic lupus Erythematosus. Patients with the systemic autoimmune diseases Sj?grens's syndrome and systemic lupus erythematosus often have autoantibodies against the intracellular protein Ro52 that is an E3 ligase dependent on the ubiquitin conjugation enzymes UBE2E1. Ro52 mediates ubiquitination through UBE2D1 in the cytoplasm and through UBE2E1 in the nucleus.

References

1. Nuber U. et al., 1996, J Biol Chem. 271 (5): 2795-800.
2. Espinosa A. et al., 2008, Exp Cell Res. 314 (20): 3605-13.
3. Anan T. et al., 1998, Genes Cells. 3 (11): 751-63.